PMID- 15584924
OWN - NLM
STAT- MEDLINE
DCOM- 20050121
LR  - 20201209
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 91
IP  - 6
DP  - 2004 Dec
TI  - Identification of Tbr-1/CASK complex target genes in neurons.
PG  - 1483-92
AB  - Tbr-1, a neuron-specific T-box transcription factor, plays a critical role in 
      brain development. Here, we performed a computational search using the 
      non-palindromic T-box binding sequence, namely the non-palindromic T-element, to 
      determine the putative downstream target genes of Tbr-1. More than 20 identified 
      genes containing the non-palindromic T-element in the 5' regulatory region were 
      found expressed in brain. Luciferase reporter assays using cultured hippocampal 
      neurons showed that overexpression of Tbr-1 and CASK-enhanced promoter activities 
      of some of these putative target genes, including NMDAR subunit 2b (NR2b), 
      glycine transporter, interleukin 7 receptor (IL-7R) and OX-2. Among these genes, 
      NR2b promoter responded strongest to overexpression of Tbr-1 and CASK. Deletion 
      of the non-palindromic T-elements from NR2b promoter impaired the induction by 
      Tbr-1 and CASK. We also examined expression of these target genes in Tbr-1 
      knockout mice, it was found that NR2b expression was consistently downregulated. 
      Similarly, both RNA and protein expression levels of NMDAR subunit 1 (NR1), which 
      also contains the non-palindromic T-elements in its 5' regulatory region, were 
      reduced in Tbr-1 knockout mice. We suggest that Tbr-1/CASK protein complex 
      regulates expression of these downstream target genes and thus modulates neuronal 
      activity and function.
FAU - Wang, Ting-Fang
AU  - Wang TF
AD  - Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
FAU - Ding, Chia-Nung
AU  - Ding CN
FAU - Wang, Guey-Shin
AU  - Wang GS
FAU - Luo, Shih-Chi
AU  - Luo SC
FAU - Lin, Yi-Ling
AU  - Lin YL
FAU - Ruan, Youlin
AU  - Ruan Y
FAU - Hevner, Robert
AU  - Hevner R
FAU - Rubenstein, John L R
AU  - Rubenstein JL
FAU - Hsueh, Yi-Ping
AU  - Hsueh YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Amino Acid Transport Systems, Neutral)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glycine Plasma Membrane Transport Proteins)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Tbr1 protein, mouse)
RN  - EC 2.7.11.1 (CASK kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.4.8 (Guanylate Kinases)
RN  - UQ4V77A8VA (antigens, CD200)
SB  - IM
MH  - Amino Acid Transport Systems, Neutral/genetics
MH  - Animals
MH  - Antigens, CD
MH  - Antigens, Surface/genetics
MH  - Base Sequence
MH  - COS Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*physiology
MH  - Cells, Cultured
MH  - Chlorocebus aethiops
MH  - DNA-Binding Proteins/*physiology
MH  - Embryo, Mammalian
MH  - *Gene Expression Regulation
MH  - Glycine Plasma Membrane Transport Proteins
MH  - Guanylate Kinases
MH  - Hippocampus/cytology
MH  - Humans
MH  - Mice
MH  - Mice, Knockout
MH  - Molecular Sequence Data
MH  - Neurons/*metabolism
MH  - Promoter Regions, Genetic/physiology
MH  - Rats
MH  - Receptors, Interleukin-7/genetics
MH  - Receptors, N-Methyl-D-Aspartate/genetics
MH  - T-Box Domain Proteins
EDAT- 2004/12/09 09:00
MHDA- 2005/01/22 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/01/22 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - JNC2845 [pii]
AID - 10.1111/j.1471-4159.2004.02845.x [doi]
PST - ppublish
SO  - J Neurochem. 2004 Dec;91(6):1483-92. doi: 10.1111/j.1471-4159.2004.02845.x.