PMID- 15584977
OWN - NLM
STAT- MEDLINE
DCOM- 20050201
LR  - 20171116
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 60
IP  - 6
DP  - 2004 Dec
TI  - Poor in vitro maturation and pro-inflammatory cytokine response of dendritic 
      cells in children at genetic risk of type 1 diabetes.
PG  - 647-52
AB  - Type 1 diabetes (T1D) has been associated with an aberrant maturation of 
      dendritic cells (DC). We studied the maturation of monocyte-derived DC in 
      children with newly diagnosed T1D and in healthy children with genetic risk for 
      T1D. Peripheral blood monocytes from children with newly diagnosed T1D (n = 12; 
      mean age 13.2 years), children with human leukocyte antigen (HLA)-risk genotype 
      of T1D (n = 7; mean age 12.7 years) and healthy children (n = 14; mean age 11.2 
      years) were in vitro differentiated into DC. Expression of HLA-DR, CD80/86 and 
      CD11c and secretion of interleukin (IL)-12, tumor necrosis factor (TNF)-alpha, 
      IL-6 and IL-10 were measured using flow cytometry. Lower percentage of DC 
      expressed CD11c and HLA-DR, and decreased production of TNF-alpha was found in 
      children with newly diagnosed T1D and in children at genetic risk when compared 
      to healthy children. Children with risk genotype also had decreased IL-12 
      production by DC. Children with T1D and children at genetic risk of T1D appear to 
      have similar aberrancies in the maturation of DC, which may predispose to 
      beta-cell autoimmunity.
FAU - Skarsvik, S
AU  - Skarsvik S
AD  - Division of Pediatrics, Department of Molecular and Clinical Medicine, Clinical 
      Research Centre, Linkoping University, Linkoping, Sweden. 
      susanne.skarsvik@imk.liu.se
FAU - Tiittanen, M
AU  - Tiittanen M
FAU - Lindstrom, A
AU  - Lindstrom A
FAU - Casas, R
AU  - Casas R
FAU - Ludvigsson, J
AU  - Ludvigsson J
FAU - Vaarala, O
AU  - Vaarala O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (Cytokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - B7-1 Antigen/immunology
MH  - CD11c Antigen/immunology
MH  - Cell Differentiation/*immunology
MH  - Cytokines/*metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Diabetes Mellitus, Type 1/*immunology/metabolism
MH  - *Genetic Predisposition to Disease
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Monocytes/immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2004/12/09 09:00
MHDA- 2005/02/03 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - SJI1521 [pii]
AID - 10.1111/j.0300-9475.2004.01521.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2004 Dec;60(6):647-52. doi: 10.1111/j.0300-9475.2004.01521.x.