PMID- 15585258
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20191210
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 26
IP  - 15
DP  - 2005 May
TI  - Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh.
PG  - 2559-66
AB  - A thin biodegradable hybrid mesh of synthetic poly(DL-lactic-co-glycolic acid) 
      (PLGA) and naturally derived collagen was used for three-dimensional culture of 
      human skin fibroblasts. The hybrid mesh was constructed by forming web-like 
      collagen microsponges in the openings of a PLGA knitted mesh. The behaviors of 
      the fibroblasts on the hybrid mesh and PLGA knitted mesh were compared. The 
      efficiency of cell seeding was much higher and the cells grew more quickly in the 
      hybrid mesh than in the PLGA mesh. The fibroblasts in the PLGA mesh grew from the 
      peripheral PLGA fibers toward the centers of the openings, while those in the 
      hybrid mesh also grew from the collagen microsponges in the openings of the mesh 
      resulting in a more homogenous growth. The proliferated cells and secreted 
      extracellular matrices were more uniformly distributed in the hybrid mesh than in 
      the PLGA mesh. Histological staining of in vitro cultured fibroblast/mesh 
      implants indicated that the fibroblasts were distributed throughout the hybrid 
      mesh and formed a uniform layer of dermal tissue having almost the same thickness 
      as that of the hybrid mesh. However, the tissue formed in the PLGA mesh was thick 
      adjacent to the PLGA fibers and thin in the center of the openings. Fibroblasts 
      cultured in the hybrid mesh were implanted in the back of nude mouse. Dermal 
      tissues were formed after 2 weeks and became epithelialized after 4 weeks. The 
      results indicate that the web-like collagen microsponges formed in the openings 
      of the PLGA knitted mesh increased the efficiency of cell seeding, improved cell 
      distribution, and therefore facilitated rapid formation of dermal tissue having a 
      uniform thickness. PLGA-collagen hybrid mesh may be useful for skin tissue 
      engineering.
FAU - Chen, Guoping
AU  - Chen G
AD  - Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, 
      Tsukuba, Ibaraki 305-0044, Japan. guoping.chen@nims.go.jp
FAU - Sato, Takashi
AU  - Sato T
FAU - Ohgushi, Hajime
AU  - Ohgushi H
FAU - Ushida, Takashi
AU  - Ushida T
FAU - Tateishi, Tetsuya
AU  - Tateishi T
FAU - Tanaka, Junzo
AU  - Tanaka J
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Absorbable Implants
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Cell Culture Techniques/methods
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen/*chemistry/ultrastructure
MH  - Fibroblasts/*cytology/physiology
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Male
MH  - Materials Testing
MH  - Mice
MH  - Mice, Nude
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Skin/*cytology
MH  - *Skin Physiological Phenomena
MH  - *Skin, Artificial
MH  - Tissue Engineering/*methods
EDAT- 2004/12/09 09:00
MHDA- 2005/06/09 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/04/27 00:00 [received]
PHST- 2004/07/20 00:00 [accepted]
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - S0142-9612(04)00686-6 [pii]
AID - 10.1016/j.biomaterials.2004.07.034 [doi]
PST - ppublish
SO  - Biomaterials. 2005 May;26(15):2559-66. doi: 10.1016/j.biomaterials.2004.07.034.