PMID- 15585309
OWN - NLM
STAT- MEDLINE
DCOM- 20050520
LR  - 20061115
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 96
IP  - 1
DP  - 2005 Jan 15
TI  - Investigation of active form of catalytic antibody light chain 41S-2-L.
PG  - 63-71
AB  - We have raised a monoclonal antibody (41S-2) against the conserved sequence, 
      RGPDRPEGIEEEGGERDRD, of human immunodeficiency virus type1 (HIV-1) envelope gp41. 
      That antibody light chain (41S-2-L) cleaves gp41-derived peptide 
      (TPRGPDRPEGIEEEGGERDRD; TP41-1) with a characteristic biphasic profile composed 
      of induction and active phases. It is considered that the conformation of 41S-2-L 
      is changed, by such as induced fitting, to move to active phase to decompose the 
      antigenic peptide during the induction phase. In order to investigate what 
      happens to 41S-2-L in the induction and active phase, the cleavage reaction of 
      the peptide by 41S-2-L was examined in detail from the viewpoint of kinetic and 
      spectroscopic analysis. The kinetic data showed that the preferable 
      conformational transition of 41S-2-L took place by the unimolecular reaction of 
      41S-2-L in the induction phase. UV-vis and fluorescence spectroscopic analysis 
      suggested that the conformational transition leads to the generation of 
      aggregates of 41S-2-L in the reacting solution, which causes the huge enhancement 
      of the catalytic activity of 41S-2-L. The nuclei of the aggregates may be formed 
      in the induction phase. The aggregates and soluble 41S-2-L are considered to be 
      in chemical equilibrium during the cleavage reaction of the antigen.
FAU - Mitsuda, Yukie
AU  - Mitsuda Y
AD  - School of Biosciences, Hiroshima Prefectural University, 562 Nanatsuka, Shobara 
      City, Hiroshima 727-0023, Japan.
FAU - Tsuruhata, Kumi
AU  - Tsuruhata K
FAU - Hifumi, Emi
AU  - Hifumi E
FAU - Takagi, Masahiro
AU  - Takagi M
FAU - Uda, Taizo
AU  - Uda T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HIV Envelope Protein gp41)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry/*immunology/metabolism
MH  - HIV Envelope Protein gp41/chemistry/*immunology/metabolism
MH  - Immunoglobulin Light Chains/chemistry/*immunology/metabolism
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peptide Fragments/chemistry/*immunology/metabolism
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
MH  - Time Factors
EDAT- 2004/12/09 09:00
MHDA- 2005/05/21 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/05/21 00:00 [received]
PHST- 2004/07/21 00:00 [revised]
PHST- 2004/07/29 00:00 [accepted]
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/05/21 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - S0165-2478(04)00218-4 [pii]
AID - 10.1016/j.imlet.2004.07.018 [doi]
PST - ppublish
SO  - Immunol Lett. 2005 Jan 15;96(1):63-71. doi: 10.1016/j.imlet.2004.07.018.