PMID- 15585925
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20191026
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 109
DP  - 2005
TI  - Designing TCR for cancer immunotherapy.
PG  - 229-56
AB  - Reprogramming T-cell populations by T-cell receptor (TCR) gene transfer is a new 
      therapeutic tool for adoptive tumor immunotherapy. Gene transfer of human 
      leukocyte antigen (HLA)-transgenic mice-derived TCR into human T-cells allows the 
      circumvention of tolerance to tumor-associated (self) antigens (TAA). This 
      chapter reports on the identification of the alpha and beta chains of the 
      heterodimeric TCR derived from a mouse T-cell clone. The related DNA fragments 
      are inserted into a retroviral vector for heterologous expression of the 
      TAA-specific TCR in human T-cells. Polymerase chain reaction (PCR)-based cloning 
      protocols are provided for the tailor-made customization of murine TCR. We 
      describe the humanization and chimerization of such TCR as well as their 
      expression in human T-cells.
FAU - Voss, Ralf-Holger
AU  - Voss RH
AD  - Department of Hematology and Oncology, Johannes Gutenberg-University, Mainz, 
      Germany.
FAU - Kuball, Jurgen
AU  - Kuball J
FAU - Theobald, Matthias
AU  - Theobald M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunotherapy/*methods
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Neoplasms/*immunology/*therapy
MH  - Receptors, Antigen, T-Cell/chemistry/genetics/*immunology/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Substrate Specificity
MH  - T-Lymphocytes/immunology/metabolism
EDAT- 2004/12/09 09:00
MHDA- 2005/03/04 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - 1-59259-862-5:229 [pii]
AID - 10.1385/1-59259-862-5:229 [doi]
PST - ppublish
SO  - Methods Mol Med. 2005;109:229-56. doi: 10.1385/1-59259-862-5:229.