PMID- 15585939
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20191026
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 109
DP  - 2005
TI  - Induction of mixed vs full chimerism to potentiate GVL effects after bone-marrow 
      transplantation.
PG  - 469-74
AB  - Graft-vs-host (GVH) alloresponses mediated by delayed donor lymphocyte infusions 
      (DLI) can occur in the absence of GVHD (graft-vs-host disease). These GVH 
      responses are confined to the lymphohematopoietic system and mediate 
      graft-vs-leukemia (GVL) reactions without causing GVHD. Although interaction of 
      donor T-cells with host-derived antigen-presenting cells (APC) is central to the 
      development of GVHD, we were able to show that this T-cell/APC interaction is 
      also critical for the induction of GVL, which can occur in the absence of GVHD if 
      donor T-cell administration is delayed to a time point after bone-marrow 
      transplant (BMT) when conditioning-induced inflammation has subsided. Induction 
      of mixed hematopoietic chimerism using the method described below allows analysis 
      of these interactions between host APC and donor T-cells.
FAU - Mapara, Markus Y
AU  - Mapara MY
AD  - Division of Hematology/Oncology, University of Pittsburgh Cancer Institute, 
      Pittsburgh, PA, USA.
FAU - Sykes, Megan
AU  - Sykes M
LA  - eng
GR  - R01 CA 79989/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology
MH  - Cell Line, Tumor
MH  - Chimera/*immunology
MH  - *Chimerism
MH  - Female
MH  - Graft vs Leukemia Effect/*immunology
MH  - Killer Cells, Natural/immunology
MH  - Mice
MH  - Phenotype
MH  - T-Lymphocytes/immunology
MH  - Tissue Donors
EDAT- 2004/12/09 09:00
MHDA- 2005/03/04 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - 1-59259-862-5:469 [pii]
AID - 10.1385/1-59259-862-5:469 [doi]
PST - ppublish
SO  - Methods Mol Med. 2005;109:469-74. doi: 10.1385/1-59259-862-5:469.