PMID- 15585940
OWN - NLM
STAT- MEDLINE
DCOM- 20050303
LR  - 20191026
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 109
DP  - 2005
TI  - Application of donor lymphocytes expressing a suicide gene for early GVL 
      induction and later control of GVH reactions after bone-marrow transplantation.
PG  - 475-86
AB  - Allogeneic hematopoietic stem cell transplantation (allo-SCT) is the treatment of 
      choice for many malignant diseases. It is recognized that the curative potential 
      of allo-SCT relates closely to the immune advantage conferred by allogeneic 
      T-lymphocytes. In allo-SCT donor T-lymphocytes favor engraftment, provide early 
      immune reconstitution, and fight the underlying malignancy--the so-called 
      graft-vs-leukemia (GVL) effect. These benefits are counterbalanced by the 
      occurrence of a life-threatening disease: graft-vs-host disease (GVHD). A suicide 
      gene encodes a protein able to convert a nontoxic prodrug into a toxic product. 
      Therefore, cells expressing the suicide gene become selectively sensitive to the 
      prodrug. The transfer of a suicide gene into donor lymphocytes could allow, upon 
      administration of the prodrug, the in vivo selective elimination of transduced 
      lymphocytes, resulting in the switch-off of GVHD, thus allowing full exploitation 
      of the curative potential of donor T-lymphocytes in the context of allo-SCT. In 
      this chapter the rationale, the materials, and the methods of the suicide-gene 
      strategy with human T-lymphocytes are described.
FAU - Bondanza, Attilio
AU  - Bondanza A
AD  - Experimental Hematology Laboratory, Cancer Immunotherapy and Gene Therapy 
      Program, San Raffaele Scientific Institute, Milan, Italy.
FAU - Ciceri, Fabio
AU  - Ciceri F
FAU - Bonini, Chiara
AU  - Bonini C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
SB  - IM
MH  - Adoptive Transfer
MH  - Bone Marrow Transplantation/*immunology
MH  - Genes, Transgenic, Suicide/*genetics
MH  - Genetic Therapy
MH  - Graft vs Host Disease/genetics/*immunology
MH  - Graft vs Leukemia Effect/genetics/*immunology
MH  - Humans
MH  - Immunotherapy
MH  - Leukemia/genetics/immunology/therapy
MH  - Lymphocytes/*immunology/*metabolism
MH  - Retroviridae/genetics
MH  - Tissue Donors
MH  - Transplantation, Homologous/immunology
EDAT- 2004/12/09 09:00
MHDA- 2005/03/04 09:00
CRDT- 2004/12/09 09:00
PHST- 2004/12/09 09:00 [pubmed]
PHST- 2005/03/04 09:00 [medline]
PHST- 2004/12/09 09:00 [entrez]
AID - 1-59259-862-5:475 [pii]
AID - 10.1385/1-59259-862-5:475 [doi]
PST - ppublish
SO  - Methods Mol Med. 2005;109:475-86. doi: 10.1385/1-59259-862-5:475.