PMID- 15588228
OWN - NLM
STAT- MEDLINE
DCOM- 20050818
LR  - 20190418
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 386
IP  - Pt 2
DP  - 2005 Mar 1
TI  - Zebrafish (Danio rerio) matrilins: shared and divergent characteristics with 
      their mammalian counterparts.
PG  - 367-79
AB  - We have cloned the cDNAs of the zebrafish (Danio rerio) members of the matrilin 
      family of extracellular adaptor proteins. In contrast to mammals, no orthologue 
      of matrilin-2 was found in zebrafish, either by RT (reverse-transcriptase) PCR 
      using degenerated primers or by screening the databases (Ensembl and NCBI); 
      however, two forms of matrilin-3, matrilin-3a and -3b, were present. The identity 
      with the mammalian matrilins is from more than 70% for the VWA (von Willebrand 
      factor A)-like domains to only 28% for the coiled-coil domains of matrilin-3a and 
      -3b. In all zebrafish matrilins we found a greater variety of splice variants 
      than in mammals, with splicing mainly affecting the number of EGF (epidermal 
      growth factor)-like repeats. The exon-intron organization is nearly identical 
      with that of mammals, and also the characteristic AT-AC intron interrupting the 
      exons coding for the coiled-coil domain is conserved. In the matrilin-3b gene a 
      unique exon codes for a proline- and serine/threonine-rich domain, possibly 
      having mucin-like properties. The matrilin-1 and -3a genes were mapped to 
      chromosome 19 and 20 respectively by the radiation hybrid method. The temporal 
      and spatial expression of zebrafish matrilins is similar to that seen in the 
      mouse. Zebrafish matrilin-4 is highly expressed as early as 24 hpf (h post 
      fertilization), whereas the other matrilins show peak expression at 72 hpf. By 
      immunostaining of whole mounts and sections, we found that matrilin-1 and -3a 
      show predominantly skeletal staining, whereas matrilin-4 is more widespread, with 
      the protein also being present in loose connective tissues and epithelia.
FAU - Ko, Ya-Ping
AU  - Ko YP
AD  - Centre for Biochemistry, Medical Faculty, University of Cologne, 
      Joseph-Stelzmann-Str. 52, D-50931 Cologne, Germany.
FAU - Kobbe, Birgit
AU  - Kobbe B
FAU - Paulsson, Mats
AU  - Paulsson M
FAU - Wagener, Raimund
AU  - Wagener R
LA  - eng
SI  - GENBANK/AJ629191
SI  - GENBANK/AJ629312
SI  - GENBANK/AJ697718
SI  - GENBANK/AJ697887
SI  - GENBANK/AJ697888
SI  - GENBANK/AJ698458
SI  - GENBANK/AJ698459
SI  - GENBANK/AJ698730
SI  - GENBANK/AJ698731
SI  - GENBANK/AJ698732
SI  - GENBANK/AJ698733
SI  - GENBANK/AJ698734
SI  - GENBANK/AJ698735
SI  - GENBANK/AJ698736
SI  - GENBANK/AJ717297
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Cartilage Oligomeric Matrix Protein)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immune Sera)
RN  - 0 (MATN1 protein, human)
RN  - 0 (Matn1 protein, mouse)
RN  - 0 (Matrilin Proteins)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TSP5 protein, human)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (matn3a protein, zebrafish)
RN  - 0 (matn3b protein, zebrafish)
RN  - 0 (matn4 protein, zebrafish)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Animals
MH  - Cartilage Oligomeric Matrix Protein
MH  - Cell Line
MH  - Cloning, Molecular/methods
MH  - Conserved Sequence/genetics
MH  - Exons/genetics
MH  - Extracellular Matrix Proteins/*genetics/immunology
MH  - Gene Expression Regulation/genetics
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Genetic Variation/*genetics
MH  - Glycoproteins/*genetics/immunology
MH  - Humans
MH  - Immune Sera/metabolism
MH  - Introns/genetics
MH  - Kidney/chemistry/cytology/embryology/metabolism
MH  - Matrilin Proteins
MH  - Molecular Sequence Data
MH  - Peptides/genetics/immunology
MH  - Radiation Hybrid Mapping/methods
MH  - Recombinant Proteins/genetics
MH  - Zebrafish/*genetics
MH  - Zebrafish Proteins/genetics/immunology
PMC - PMC1134802
EDAT- 2004/12/14 09:00
MHDA- 2005/08/19 09:00
PMCR- 2005/09/01
CRDT- 2004/12/14 09:00
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/08/19 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
PHST- 2005/09/01 00:00 [pmc-release]
AID - BJ20041486 [pii]
AID - bj3860367 [pii]
AID - 10.1042/BJ20041486 [doi]
PST - ppublish
SO  - Biochem J. 2005 Mar 1;386(Pt 2):367-79. doi: 10.1042/BJ20041486.