PMID- 15588472
OWN - NLM
STAT- MEDLINE
DCOM- 20050314
LR  - 20190917
IS  - 1472-6483 (Print)
IS  - 1472-6483 (Linking)
VI  - 9
IP  - 5
DP  - 2004 Nov
TI  - Clinical aspects of preimplantation genetic diagnosis for single gene disorders 
      combined with HLA typing.
PG  - 529-32
AB  - Preimplantation genetic diagnosis (PGD) for single gene disorders combined with 
      human leukocyte antigen (HLA) matching has recently emerged as a therapeutic tool 
      for stem cell transplantation in couples bearing an affected offspring. There may 
      exist, however, several patient- or cycle-specific limitations for certain 
      couples. This article documents data regarding experience of single gene 
      disorders combined with HLA matching obtained at Istanbul Memorial Hospital, 
      Turkey. The data were obtained from 20 couples undergoing 26 PGD-HLA cycles for 
      thalassaemia (n = 23), Wiscott-Aldrich syndrome (n = 1) and acute lymphoblastic 
      leukaemia (n = 2). A total of 206 embryos was biopsied on day 3 of embryo 
      development and subsequently analysed. After the analysis, 26 (12.6%) of them 
      were found to be both healthy and HLA compatible. In 16 embryo transfers 
      performed, seven (43.7%) clinical pregnancies were obtained, one of which 
      resulted in miscarriage. Ten of the 26 cycles started (38.4%) were cancelled due 
      to a lack of suitable (mutation-free and/or HLA-compatible) embryos. The data 
      suggest that application of PGD in combination with HLA typing is a promising 
      therapeutic tool for an affected sibling.
FAU - Kahraman, S
AU  - Kahraman S
AD  - Istanbul Memorial Hospital, ART, Reproductive Endocrinology and Genetics Unit, 
      Piyalepasa Bulvari, 80270 Okmeydani, Istanbul, Turkey. skahraman@superonline.com
FAU - Karlikaya, G
AU  - Karlikaya G
FAU - Sertyel, S
AU  - Sertyel S
FAU - Karadayi, H
AU  - Karadayi H
FAU - Findikli, N
AU  - Findikli N
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
SB  - IM
MH  - Adult
MH  - Female
MH  - Genetic Diseases, Inborn/*diagnosis/genetics
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Infertility/*diagnosis/genetics
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - *Preimplantation Diagnosis
EDAT- 2004/12/14 09:00
MHDA- 2005/03/15 09:00
CRDT- 2004/12/14 09:00
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/03/15 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
AID - S1472-6483(10)61637-9 [pii]
AID - 10.1016/s1472-6483(10)61637-9 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2004 Nov;9(5):529-32. doi: 10.1016/s1472-6483(10)61637-9.