PMID- 15588920
OWN - NLM
STAT- MEDLINE
DCOM- 20050224
LR  - 20191210
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 206
IP  - 2
DP  - 2005 Jan 15
TI  - Qualitative and quantitative evaluation of a local lymph node assay based on ex 
      vivo interleukin-2 production.
PG  - 285-98
AB  - The local lymph node assay (LLNA) is a regular method for the detection of 
      sensitizing chemicals in mice which measures the incorporation of tritiated 
      thymidine in lymph node cells. We have evaluated an alternative to this method 
      based on the interleukin-2 (IL-2) production of lymph node cells. At the mRNA 
      level, no change in the IL-2 gene expression level was detected by real-time PCR 
      analysis. At the protein level, various experimental conditions were checked in 
      order to improve the irritant versus sensitizer discrimination with a restricted 
      set of prototypic compounds. In particular, the use of phytohemagglutinin A (PHA) 
      in an ex vivo cell culture step showed an improvement of both signal and 
      discrimination. In these optimised conditions, a panel of irritants and 
      potency-graded sensitizers was used to assess the performance of the modified 
      method. IFN-gamma production was used as a positive control. For each compound, a 
      dose-response was performed and stimulation indexes (SI) were determined. 
      Effective concentrations (EC) for each sensitizers were then extracted and 
      compared to the literature data of the regular LLNA. The IL-2-based LLNA showed 
      similar performances at both qualitative and quantitative levels compared to 
      regular LLNA.
FAU - Azam, Philippe
AU  - Azam P
AD  - AFSSAPS, Unite BCM, 635 Rue de la Garenne, 34740 Vendargues, France.
FAU - Peiffer, Jean-Luc
AU  - Peiffer JL
FAU - Ourlin, Jean-Claude
AU  - Ourlin JC
FAU - Bonnet, Pierre-Antoine
AU  - Bonnet PA
FAU - Tissier, Marie-Helene
AU  - Tissier MH
FAU - Vian, Laurence
AU  - Vian L
FAU - Fabre, Isabelle
AU  - Fabre I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Interleukin-2)
RN  - 0 (Irritants)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animal Testing Alternatives
MH  - Animals
MH  - Dermatitis, Allergic Contact/etiology/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Interferon-gamma/genetics/immunology
MH  - Interleukin-2/*biosynthesis/genetics
MH  - Irritants/*toxicity
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/*drug effects/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/12/14 09:00
MHDA- 2005/02/25 09:00
CRDT- 2004/12/14 09:00
PHST- 2004/05/20 00:00 [received]
PHST- 2004/09/15 00:00 [accepted]
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/02/25 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
AID - S0300-483X(04)00564-5 [pii]
AID - 10.1016/j.tox.2004.09.003 [doi]
PST - ppublish
SO  - Toxicology. 2005 Jan 15;206(2):285-98. doi: 10.1016/j.tox.2004.09.003.