PMID- 15591214
OWN - NLM
STAT- MEDLINE
DCOM- 20051108
LR  - 20071115
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 25
IP  - 3
DP  - 2005 Mar
TI  - Oxidized phospholipids trigger atherogenic inflammation in murine arteries.
PG  - 633-8
AB  - OBJECTIVE: Lipoprotein-derived phospholipid oxidation products have been 
      implicated as candidate triggers of the inflammatory process in atherosclerosis. 
      However, in vivo evidence regarding the impact of oxidized phospholipids on the 
      artery wall thus far has been elusive. Therefore, the aim of this study was to 
      investigate if structurally defined oxidized phospholipids induce expression of 
      atherogenic chemokines and monocyte adhesion in intact murine arteries. METHODS 
      AND RESULTS: To model the accumulation of oxidized phospholipids in the arterial 
      wall, oxidized 1-palmitoyl-2-arachidonoyl-sn-3-glycero-phosphorylcholine (OxPAPC) 
      was topically applied to carotid arteries in mice using pluronic gel. Using 
      quantitative reverse-transcriptase polymerase chain reaction (PCR) and 
      immunohistochemistry, we show that OxPAPC induced a set of 
      atherosclerosis-related genes, including monocyte chemotactic protein 1 (MCP-1) 
      and keratinocyte-derived chemokine (KC), tissue factor (TF), interleukin 6 
      (IL-6), heme oxygenase 1 (HO-1), and early growth response 1 (EGR-1). 
      OxPAPC-regulated chemokines were also expressed in atherosclerotic lesions of 
      apolipoprotein E-deficient (ApoE-/-) mice. In isolated perfused carotid arteries, 
      OxPAPC triggered rolling and firm adhesion of monocytes in a P-selectin and 
      KC-dependent manner. CONCLUSIONS: Oxidized phospholipids contribute to vascular 
      inflammation in murine arteries in vivo, initiating atherogenic chemokine 
      expression that leads to monocyte adhesion; therefore, they can be regarded as 
      triggers of the inflammatory process in atherosclerosis.
FAU - Furnkranz, Alexander
AU  - Furnkranz A
AD  - Department of Vascular Biology and Thrombosis Research, University of Vienna, 
      Austria.
FAU - Schober, Andreas
AU  - Schober A
FAU - Bochkov, Valery N
AU  - Bochkov VN
FAU - Bashtrykov, Pavel
AU  - Bashtrykov P
FAU - Kronke, Gerhard
AU  - Kronke G
FAU - Kadl, Alexandra
AU  - Kadl A
FAU - Binder, Bernd R
AU  - Binder BR
FAU - Weber, Christian
AU  - Weber C
FAU - Leitinger, Norbert
AU  - Leitinger N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041209
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Apolipoproteins E)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl1 protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Egr1 protein, mouse)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (P-Selectin)
RN  - 0 (Phospholipids)
RN  - 0 (Transcription Factors)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - Animals
MH  - Apolipoproteins E/genetics
MH  - Carotid Arteries/immunology/metabolism
MH  - Carotid Artery Diseases/*immunology/*metabolism/physiopathology
MH  - Chemokine CXCL1
MH  - Chemokines/metabolism
MH  - Chemokines, CXC/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Early Growth Response Protein 1
MH  - Gene Expression/immunology
MH  - Immediate-Early Proteins/genetics
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Leukocyte Rolling/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Monocytes/cytology/immunology
MH  - Oxidation-Reduction
MH  - P-Selectin/metabolism
MH  - Phospholipids/*metabolism
MH  - Thromboplastin/genetics
MH  - Transcription Factors/genetics
MH  - Vasculitis/*immunology/*metabolism/physiopathology
EDAT- 2004/12/14 09:00
MHDA- 2005/11/09 09:00
CRDT- 2004/12/14 09:00
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/11/09 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
AID - 01.ATV.0000153106.03644.a0 [pii]
AID - 10.1161/01.ATV.0000153106.03644.a0 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):633-8. doi: 
      10.1161/01.ATV.0000153106.03644.a0. Epub 2004 Dec 9.