PMID- 15592516
OWN - NLM
STAT- MEDLINE
DCOM- 20050314
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 24
IP  - 7
DP  - 2005 Feb 10
TI  - HTLV-I Tax induces and associates with Crk-associated substrate lymphocyte type 
      (Cas-L).
PG  - 1262-71
AB  - Crk-associated substrate lymphocyte type (Cas-L) is a docking protein that is 
      heavily tyrosine phosphorylated by the engagement of beta1 integrins in T cells. 
      In the present study, we attempted to evaluate the role of Cas-L in the 
      pathophysiology of adult T-cell leukemia (ATL). Examination of peripheral blood 
      mononuclear cells from ATL patients as well as ATL-derived T cell lines showed an 
      elevation of Cas-L in these cells. We showed that tyrosine phosphorylation as 
      well as expression of Cas-L was markedly elevated through the induction of human 
      T-lymphotropic virus type I (HTLV-I) Tax in JPX-9 cells, with these cells showing 
      marked motile behavior on the ligands for integrins. We next performed yeast 
      two-hybrid screening of cDNA library from an HTLV-I-transformed T cell line, 
      which resulted in the identification of Tax as a putative binding partner for 
      Cas-L. Co-precipitation experiments revealed that the serine-rich region of Cas-L 
      might serve as the binding site with the highest affinity for Tax. 
      Co-localization study showed that Tax and Cas-L partly merged in the cytoplasm. 
      Finally, we showed that exogenous Cas-L inhibited Tax-mediated transactivation of 
      nuclear factor kappaB (NF-kappaB), while Tax-independent activation of NF-kappaB 
      remained intact, hence indicating that Cas-L might specifically regulate 
      Tax-NF-kappaB pathway.
FAU - Iwata, Satoshi
AU  - Iwata S
AD  - Division of Clinical Immunology, Advanced Clinical Research Center, Institute of 
      Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 
      108-8639, Japan.
FAU - Souta-Kuribara, Akiko
AU  - Souta-Kuribara A
FAU - Yamakawa, Akio
AU  - Yamakawa A
FAU - Sasaki, Takahiro
AU  - Sasaki T
FAU - Shimizu, Takatsune
AU  - Shimizu T
FAU - Hosono, Osamu
AU  - Hosono O
FAU - Kawasaki, Hiroshi
AU  - Kawasaki H
FAU - Tanaka, Hirotoshi
AU  - Tanaka H
FAU - Dang, Nam H
AU  - Dang NH
FAU - Watanabe, Toshiki
AU  - Watanabe T
FAU - Arima, Naomichi
AU  - Arima N
FAU - Morimoto, Chikao
AU  - Morimoto C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Gene Products, tax)
RN  - 0 (NEDD9 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Phosphoproteins)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Cadmium Chloride/pharmacology
MH  - Cell Line, Transformed
MH  - Cell Movement/genetics/physiology
MH  - Cytosol/chemistry
MH  - Gene Products, tax/analysis/*metabolism
MH  - Human T-lymphotropic virus 1/metabolism
MH  - Humans
MH  - Immunoprecipitation
MH  - Leukemia, T-Cell/*virology
MH  - Leukocytes, Mononuclear/chemistry/drug effects/physiology
MH  - NF-kappa B/physiology
MH  - Phosphoproteins/analysis/metabolism/*physiology
MH  - Phosphorylation
MH  - Transcriptional Activation/physiology
MH  - Two-Hybrid System Techniques
MH  - Up-Regulation
EDAT- 2004/12/14 09:00
MHDA- 2005/03/15 09:00
CRDT- 2004/12/14 09:00
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/03/15 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
AID - 1208261 [pii]
AID - 10.1038/sj.onc.1208261 [doi]
PST - ppublish
SO  - Oncogene. 2005 Feb 10;24(7):1262-71. doi: 10.1038/sj.onc.1208261.