PMID- 15592888
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20220228
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 150
IP  - 4
DP  - 2005 Apr
TI  - Presence of HTLV-I Tax protein in cerebrospinal fluid from HAM/TSP patients.
PG  - 743-53
AB  - Infection with human T-cell lymphotropic virus type I (HTLV-I) have been 
      associated with the development of the HTLV-I-associated myelopathy/Tropical 
      Spastic Paraparesis (HAM/TSP). The disease affects the pyramidal tract at the 
      distal segments of spinal cord, generating a spastic paraparesis. We studied the 
      presence of Tax protein in cerebrospinal fluid cells and spinal fluid (CSF) of 35 
      Chilean patients: 22 HAM/TSP patients (15 HTLV-I-seropositives, and 7 
      seronegatives), and 13 controls (9 PSP and 4 CJD non-infected patients). Tax 
      antigens were evaluated with monoclonal antibodies reacting with Tax by 
      immunofluorescence and ELISA assays in cerebrospinal fluid cells and CSF, 
      respectively. Proviral was evaluated by PCR of tax gene in cerebrospinal fluid 
      cells. Tax antigen was detected in CSF and lymphocytes of CSF from 4 and 12 
      HAM/TSP patients, respectively. Lymphocytes of CSF of 8 HAM/TSP (6 seropositives 
      and 2 seronegatives) showed the presence of tax gene. These results show that 
      cells of CSF from HAM/TSP patients are able to express and export Tax protein 
      towards the CSF. This is the first report of the presence of Tax protein in 
      cerebrospinal fluid cells and CSF from HAM/TSP HTLV-I seronegative patients.
FAU - Cartier, L
AU  - Cartier L
AD  - Department of Neurological Sciences of Medicine Faculty, Hospital del Salvador, 
      University of Chile, Santiago, Chile.
FAU - Ramirez, E
AU  - Ramirez E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041210
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Gene Products, tax)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - Antigens, Viral/cerebrospinal fluid
MH  - Chile
MH  - Creutzfeldt-Jakob Syndrome/cerebrospinal fluid
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Products, tax/*cerebrospinal fluid
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/*cerebrospinal fluid/physiopathology/virology
MH  - Reference Values
EDAT- 2004/12/14 09:00
MHDA- 2005/06/29 09:00
CRDT- 2004/12/14 09:00
PHST- 2004/06/02 00:00 [received]
PHST- 2004/10/08 00:00 [accepted]
PHST- 2004/12/14 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2004/12/14 09:00 [entrez]
AID - 10.1007/s00705-004-0443-3 [doi]
PST - ppublish
SO  - Arch Virol. 2005 Apr;150(4):743-53. doi: 10.1007/s00705-004-0443-3. Epub 2004 Dec 
      10.