PMID- 1559367
OWN - NLM
STAT- MEDLINE
DCOM- 19920508
LR  - 20190706
IS  - 0090-3493 (Print)
IS  - 0090-3493 (Linking)
VI  - 20
IP  - 4
DP  - 1992 Apr
TI  - Tumor necrosis factor-alpha causes myocardial depression in guinea pigs.
PG  - 523-7
AB  - BACKGROUND AND METHODS: Tumor necrosis factor-alpha (TNF-alpha) is an important 
      mediator of metabolic and cardiovascular derangements in sepsis and 
      endotoxicosis. We tested the hypothesis that TNF-alpha causes myocardial 
      depression and alters the cardiac responsiveness to administered norepinephrine. 
      Albino Hartley guinea pigs (n = 32) of either sex were injected iv with saline 
      (1.5 mL) or recombinant human TNF-alpha (1 mg/kg). At 6, 24, or 72 hrs after 
      injection, atria were harvested, split, connected to force displacement x 
      transducer-amplifier-recorder systems and maintained in vitro in oxygenated 37.5 
      degrees C Krebs-Henseleit buffer. RESULTS: Maximal left atrial force of 
      contraction and maximal left atrial velocity of contraction were decreased in the 
      TNF-alpha treated animals compared with controls (p less than .05), irrespective 
      of time after TNF-alpha injection. There were no differences between groups for 
      left atrial maximal velocity of relaxation and right atrial rate. The 
      norepinephrine concentration that elicited a 50% maximal left atrial contractile 
      response (ED50) was higher in TNF-alpha treated animals compared with controls (p 
      less than .05). Maximal left atrial force of contraction, maximal right atrial 
      rate, and right atrial ED50 were similar in the two groups. CONCLUSIONS: These 
      results indicate that TNF-alpha injected in vivo causes in vitro myocardial 
      depression and alters cardiac responsiveness to norepinephrine.
FAU - Heard, S O
AU  - Heard SO
AD  - Department of Anesthesiology, University of Massachusetts Medical Center, 
      Worcester.
FAU - Perkins, M W
AU  - Perkins MW
FAU - Fink, M P
AU  - Fink MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Crit Care Med
JT  - Critical care medicine
JID - 0355501
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Depression, Chemical
MH  - Female
MH  - Guinea Pigs
MH  - Heart Rate/*drug effects
MH  - In Vitro Techniques
MH  - Male
MH  - Myocardial Contraction/*drug effects
MH  - Norepinephrine/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1097/00003246-199204000-00015 [doi]
PST - ppublish
SO  - Crit Care Med. 1992 Apr;20(4):523-7. doi: 10.1097/00003246-199204000-00015.