PMID- 15596412
OWN - NLM
STAT- MEDLINE
DCOM- 20050215
LR  - 20071115
IS  - 1521-6616 (Print)
IS  - 1521-6616 (Linking)
VI  - 114
IP  - 1
DP  - 2005 Jan
TI  - Elevated monocyte chemotactic proteins 1, 2, and 3 in pulmonary alveolar 
      proteinosis are associated with chemokine receptor suppression.
PG  - 79-85
AB  - Pulmonary alveolar proteinosis (PAP) is a rare autoimmune lung disease 
      characterized by abnormal surfactant accumulation within alveolar macrophages, 
      and circulating auto-antibodies against granulocyte-macrophage colony stimulating 
      factor (GM-CSF) resulting in functional GM-CSF deficiency. Monocyte/macrophage 
      chemotactic protein-1 (MCP-1) is elevated in PAP, suggesting association with the 
      pathophysiology. Because PAP has been associated with inflammatory pulmonary 
      changes, we hypothesized that other MCP family chemokines would be present and 
      that Chemokine Chemotaxis Receptor 2 (CCR2) would be elevated on PAP mononuclear 
      cells. Here we show for the first time that MCP-2 and MCP-3, like MCP-1, are 
      highly elevated in PAP. We also confirm that PAP alveolar macrophages and not 
      epithelial cells produce MCP-1, and that MCP-1 from PAP lung has functional 
      chemoattractant activity. Surprisingly, CCR2 expression is diminished in PAP 
      lymphocytes and alveolar macrophages compared to controls. Further, MCP-1 from 
      PAP lung suppresses CCR2 expression in vitro, suggesting that in PAP, MCP-1 
      participates in an autocrine regulatory network in vivo.
FAU - Bonfield, Tracey L
AU  - Bonfield TL
AD  - Department of Pulmonary, Allergy and Critical Care Medicine, The Cleveland Clinic 
      Foundation, Cleveland, OH 44195, USA.
FAU - John, Nejimol
AU  - John N
FAU - Malur, Anagha
AU  - Malur A
FAU - Barna, Barbara P
AU  - Barna BP
FAU - Culver, Daniel A
AU  - Culver DA
FAU - Kavuru, Mani S
AU  - Kavuru MS
FAU - Thomassen, Mary Jane
AU  - Thomassen MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Immunol
JT  - Clinical immunology (Orlando, Fla.)
JID - 100883537
RN  - 0 (CCL7 protein, human)
RN  - 0 (CCL8 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL7)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bronchoalveolar Lavage
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CCL7
MH  - Chemokine CCL8
MH  - Chemokines/physiology
MH  - Cytokines/*metabolism
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Macrophages, Alveolar/immunology
MH  - Male
MH  - Middle Aged
MH  - Monocyte Chemoattractant Proteins/*metabolism
MH  - Pulmonary Alveolar Proteinosis/*immunology
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/biosynthesis/*physiology
EDAT- 2004/12/15 09:00
MHDA- 2005/02/16 09:00
CRDT- 2004/12/15 09:00
PHST- 2004/08/04 00:00 [received]
PHST- 2004/09/13 00:00 [accepted]
PHST- 2004/12/15 09:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2004/12/15 09:00 [entrez]
AID - S1521-6616(04)00290-6 [pii]
AID - 10.1016/j.clim.2004.09.004 [doi]
PST - ppublish
SO  - Clin Immunol. 2005 Jan;114(1):79-85. doi: 10.1016/j.clim.2004.09.004.