PMID- 15597191
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20151119
IS  - 0340-2061 (Print)
IS  - 0340-2061 (Linking)
VI  - 209
IP  - 2
DP  - 2004 Dec
TI  - Differences in development and cellular composition between neuronal cultures of 
      rat accessory and main olfactory bulbs.
PG  - 129-36
AB  - We previously established a primary culture system of the accessory olfactory 
      bulb (AOB) to investigate the functional roles of individual types of neuron in 
      pheromonal signal processing. However, the detailed characteristics of cultured 
      AOB neurons were not yet apparent. In the present study, we address the 
      cytological aspects of cultured AOB neurons using immunocytochemical staining 
      methods. Cultured AOB neurons were compared with cultured main olfactory bulb 
      (MOB) neurons in neuronal composition, maturational time course, and cell size. 
      The number of total neurons, measured by microtubule-associated protein 2 (MAP2) 
      immunostaining, progressively decreased, and glutamic acid decarboxylase positive 
      (GAD+) interneurons were scarcely changed in their number in both AOB and MOB 
      cultures over the culture periods. In contrast, the number of tyrosine 
      hydroxylase positive (TH+) neurons in AOB cultures showed a slight, but 
      significant, increase over time in culture, while those in MOB cultures 
      remarkably decreased. The numbers of total neurons and GAD+ neurons were 
      significantly greater in AOB cultures than in MOB cultures at all investigated 
      time points. However, the numbers of TH+ neurons were lower at 7 days in vitro 
      (DIV) and greater at 21 DIV in AOB cultures than in MOB cultures. The somatic 
      sizes of all types of neurons at 14 DIV were significantly larger in AOB cultures 
      than in MOB cultures. Furthermore, the frequency distributions of somatic sizes 
      of total, GAD+, and TH+ neurons were significantly different between AOB and MOB 
      cultures. These subtle differences in vitro may reflect in vivo differences 
      between the AOB and MOB.
FAU - Muramoto, Kazuyo
AU  - Muramoto K
AD  - Department of Integrative Physiology, Kochi Medical School, Kohasu, Nankoku, 
      783-8505, Kochi, Japan. tkazuyo@med.kochi-u.ac.jp
FAU - Kato-Negishi, Midori
AU  - Kato-Negishi M
FAU - Kuroda, Yoichiro
AU  - Kuroda Y
FAU - Kaba, Hideto
AU  - Kaba H
FAU - Ichikawa, Masumi
AU  - Ichikawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041119
PL  - Germany
TA  - Anat Embryol (Berl)
JT  - Anatomy and embryology
JID - 7505194
RN  - 0 (Biomarkers)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Count
MH  - Cell Differentiation/*physiology
MH  - Cell Size
MH  - Cells, Cultured
MH  - Dopamine/metabolism
MH  - Glutamate Decarboxylase/metabolism
MH  - Immunohistochemistry
MH  - Interneurons/cytology/metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/*cytology/metabolism
MH  - Olfactory Bulb/*cytology/*embryology/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2004/12/15 09:00
MHDA- 2005/08/03 09:00
CRDT- 2004/12/15 09:00
PHST- 2004/09/07 00:00 [accepted]
PHST- 2004/12/15 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2004/12/15 09:00 [entrez]
AID - 10.1007/s00429-004-0432-z [doi]
PST - ppublish
SO  - Anat Embryol (Berl). 2004 Dec;209(2):129-36. doi: 10.1007/s00429-004-0432-z. Epub 
      2004 Nov 19.