PMID- 15599055
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20191109
IS  - 1341-7568 (Print)
IS  - 1341-7568 (Linking)
VI  - 79
IP  - 5
DP  - 2004 Oct
TI  - Unusual distribution of mitochondrial large subunit rRNA in the cytosol during 
      conjugation in Tetrahymena thermophila.
PG  - 255-62
AB  - The distribution of mitochondria during conjugation of the ciliated protozoan 
      Tetrahymena thermophila was surveyed using a mitochondrial stain and fluorescence 
      in situ hybridization (FISH). When the mitochondria-specific stain, Mito-Tracker, 
      was used, the majority of mitochondria were detected in the cortex; their 
      distribution was not changed during conjugation. On the other hand, FISH using 
      mitochondrial large subunit (LSU) rRNA as a probe showed an unusual distribution 
      of signals during conjugation. Unexpectedly, the signals were detected throughout 
      the cytoplasm of conjugating cells. These signals were not observed in pre-mating 
      cells and in exconjugants. The cytosolic localization of mitochondrial rRNA was 
      supported by northern blot analysis using post-mitochondrial RNA fraction at the 
      later stages of conjugation. These observations suggest selective mitochondrial 
      breakdown or transport of LSU rRNA into cytosol. The biological significance of 
      the conjugation-specific appearance of the cytosolic mitochondrial rRNA is 
      discussed.
FAU - Kobayashi, Takashi
AU  - Kobayashi T
AD  - Department of Biology, Faculty of Science, Kanazawa University, Kakuma, Kanazawa 
      920-1192, Japan. tacobys@aichi-med-u.ac.jp
FAU - Endoh, Hiroshi
AU  - Endoh H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Genes Genet Syst
JT  - Genes & genetic systems
JID - 9607822
RN  - 0 (RNA, Mitochondrial)
RN  - 0 (RNA, Protozoan)
RN  - 0 (RNA, Ribosomal)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - *Conjugation, Genetic
MH  - Cytosol/*metabolism/ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Mitochondria/*metabolism/ultrastructure
MH  - RNA/metabolism
MH  - RNA, Mitochondrial
MH  - RNA, Protozoan/*metabolism
MH  - RNA, Ribosomal/*metabolism
MH  - Tetrahymena thermophila/*genetics/physiology/ultrastructure
EDAT- 2004/12/16 09:00
MHDA- 2005/05/25 09:00
CRDT- 2004/12/16 09:00
PHST- 2004/12/16 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2004/12/16 09:00 [entrez]
AID - JST.JSTAGE/ggs/79.255 [pii]
AID - 10.1266/ggs.79.255 [doi]
PST - ppublish
SO  - Genes Genet Syst. 2004 Oct;79(5):255-62. doi: 10.1266/ggs.79.255.