PMID- 15599101
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20190911
IS  - 1347-8613 (Print)
IS  - 1347-8613 (Linking)
VI  - 96
IP  - 4
DP  - 2004 Dec
TI  - Malfunction of vascular control in lifestyle-related diseases: formation of 
      systemic hemoglobin-nitric oxide complex (HbNO) from dietary nitrite.
PG  - 395-400
AB  - Nitric oxide (NO) has many physiological functions. It is believed to be produced 
      from L-arginine by nitric oxide synthase (NOS), and nitrite and nitrate are waste 
      forms of it. By the way, nitrate and nitrite are abundant in vegetables and 
      fruits, especially leafy vegetables and pickled vegetables. Orally-ingested 
      nitrate is changed to nitrite by micro-organelles living in the hypopharynx area, 
      and nitrite is expected to change to NO in the stomach due to its low pH. Indeed, 
      some researchers reported that NO is produced in the gastric cavity, although few 
      reports mentioned the physiological meanings of this NO formation. Therefore, we 
      investigated whether the nitrite-derived NO can shift to the circulation and acts 
      like NOS-derived NO does in tissues. We adopted a stable isotope of nitrite 
      (15NO2-) in order to distinguish between the endogenous nitrite and the 
      exogenously administered one and measured nitrosyl hemoglobin (HbNO) as an index 
      of circulating NO using electron paramagnetic resonance spectroscopy. It appeared 
      that the oral administration of 15N-nitrite formed the Hb15NO in rat blood and 
      decreased the blood pressure of chronic L-NAME treated rats. Our findings suggest 
      that the intake of nitrite (or nitrate)-rich foods such as vegetables and fruits 
      would alter the systemic HbNO dynamism, resulting in the improvement of 
      cardiovascular diseases.
FAU - Tsuchiya, Koichiro
AU  - Tsuchiya K
AD  - Department of Clinical Pharmacology, Subdivision of Clinical Pharmaceutical 
      Sciences, Institute of Health Biosciences, The University of Tokushima Graduate 
      School, 1-78 Sho-machi, Tokushima 770-8505, Japan. tsuchiya@ph.tokushima-u.ac.jp
FAU - Takiguchi, Yoshiharu
AU  - Takiguchi Y
FAU - Okamoto, Masumi
AU  - Okamoto M
FAU - Izawa, Yuki
AU  - Izawa Y
FAU - Kanematsu, Yasuhisa
AU  - Kanematsu Y
FAU - Yoshizumi, Masanori
AU  - Yoshizumi M
FAU - Tamaki, Toshiaki
AU  - Tamaki T
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20041210
PL  - Japan
TA  - J Pharmacol Sci
JT  - Journal of pharmacological sciences
JID - 101167001
RN  - 0 (Hemoglobins)
RN  - 0 (Nitrites)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Diet
MH  - Dose-Response Relationship, Drug
MH  - Electron Spin Resonance Spectroscopy
MH  - Hemoglobins/*metabolism
MH  - Kidney/metabolism
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*metabolism
MH  - Nitrites/*metabolism
MH  - Rats
RF  - 56
EDAT- 2004/12/16 09:00
MHDA- 2005/04/29 09:00
CRDT- 2004/12/16 09:00
PHST- 2004/12/16 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2004/12/16 09:00 [entrez]
AID - JST.JSTAGE/jphs/FMJ04006X3 [pii]
AID - 10.1254/jphs.fmj04006x3 [doi]
PST - ppublish
SO  - J Pharmacol Sci. 2004 Dec;96(4):395-400. doi: 10.1254/jphs.fmj04006x3. Epub 2004 
      Dec 10.