PMID- 15601848
OWN - NLM
STAT- MEDLINE
DCOM- 20050118
LR  - 20181201
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 25
IP  - 1
DP  - 2005 Jan
TI  - Cyclin-dependent kinase activity is required for progesterone receptor function: 
      novel role for cyclin A/Cdk2 as a progesterone receptor coactivator.
PG  - 264-77
AB  - Our studies examining the role of the cell cycle-regulated kinase cyclin A/Cdk2 
      in progesterone receptor (PR) action have demonstrated that cyclin-dependent 
      kinase activity is required for PR function and that cyclin A/Cdk2 functions as a 
      PR coactivator. Although Cdk2 can phosphorylate PR, elimination of these 
      phosphorylation sites has little effect on the ability of cyclin A/Cdk2 to 
      stimulate PR activity. PR interacts with cyclin A and recruits cyclin A/Cdk2 to 
      progestin-responsive promoters, stimulating transcription. Inhibition of Cdk2 
      activity abolishes progesterone-dependent activation of PR target genes in part 
      through inhibition of PR-dependent recruitment of steroid receptor coactivator 1 
      (SRC-1) and subsequent histone H4 acetylation at the target promoter. In vitro 
      studies revealed that the interaction between SRC-1 and PR is dependent upon 
      phosphorylation of SRC-1. This heretofore-unknown mechanism provides a potential 
      means for integrating the regulation of PR activity with cell cycle progression. 
      Moreover, the ability of PR to recruit cyclin A/Cdk2 to target promoters provides 
      locally elevated levels of kinase, which can preferentially facilitate 
      phosphorylation-dependent interactions and enzymatic activities of coactivators 
      at the target promoter.
FAU - Narayanan, Ramesh
AU  - Narayanan R
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine, One 
      Baylor Plaza, Houston, TX 77030, USA.
FAU - Adigun, Abayomi A
AU  - Adigun AA
FAU - Edwards, Dean P
AU  - Edwards DP
FAU - Weigel, Nancy L
AU  - Weigel NL
LA  - eng
GR  - R01 CA057539/CA/NCI NIH HHS/United States
GR  - T32 HD007165/HD/NICHD NIH HHS/United States
GR  - R01 CA 57539/CA/NCI NIH HHS/United States
GR  - T32 HD 07165/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cyclin A)
RN  - 0 (Histones)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Purines)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Transcription Factors)
RN  - 0ES1C2KQ94 (Roscovitine)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (CDK2 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Blotting, Western
MH  - CDC2-CDC28 Kinases/*metabolism
MH  - COS Cells
MH  - Cell Cycle
MH  - Cell Line
MH  - Chromatin Immunoprecipitation
MH  - Cyclin A/*metabolism
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - Genes, Reporter
MH  - Genetic Vectors
MH  - Glutathione Transferase/metabolism
MH  - HeLa Cells
MH  - Histone Acetyltransferases
MH  - Histones/metabolism
MH  - Humans
MH  - Immunoprecipitation
MH  - Lysine/chemistry
MH  - Models, Genetic
MH  - Nuclear Receptor Coactivator 1
MH  - Phosphorylation
MH  - Plasmids/metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Biosynthesis
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Purines/pharmacology
MH  - Receptors, Progesterone/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Roscovitine
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic
MH  - Transcriptional Activation
MH  - Transfection
MH  - Two-Hybrid System Techniques
PMC - PMC538783
EDAT- 2004/12/17 09:00
MHDA- 2005/01/19 09:00
PMCR- 2005/01/01
CRDT- 2004/12/17 09:00
PHST- 2004/12/17 09:00 [pubmed]
PHST- 2005/01/19 09:00 [medline]
PHST- 2004/12/17 09:00 [entrez]
PHST- 2005/01/01 00:00 [pmc-release]
AID - 25/1/264 [pii]
AID - 1330-04 [pii]
AID - 10.1128/MCB.25.1.264-277.2005 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2005 Jan;25(1):264-77. doi: 10.1128/MCB.25.1.264-277.2005.