PMID- 15601968
OWN - NLM
STAT- MEDLINE
DCOM- 20050317
LR  - 20131121
IS  - 1071-7323 (Print)
IS  - 1071-7323 (Linking)
VI  - 12
IP  - 11
DP  - 2004 Nov
TI  - n-3 fatty acids modulate T-cell calcium signaling in obese macrosomic rats.
PG  - 1744-53
AB  - OBJECTIVE: We investigated the effects of a diet containing EPAX-7010, rich in 
      PUFAs such as eicosapentaenoic acid [20:5(n-3)] and docosahexaenoic acid 
      [22:6(n-3)], i.e., a PUFA/EPAX regimen, on T-cell activation in diabetic pregnant 
      rats and their obese pups. RESEARCH METHODS AND PROCEDURES: Mild hyperglycemia in 
      pregnant rats was induced by intraperitoneal injection of streptozotocin on Day 5 
      of gestation. T-cell blastogenesis was assayed by using (3)H-thymidine, whereas 
      intracellular free calcium concentrations ([Ca(2+)]i) were measured by using 
      Fura-2 in diabetic pregnant rats and their obese offspring. RESULTS: 
      Concavalin-A-stimulated T-cell proliferation was decreased in both pregnant 
      diabetic rats and their obese pups as compared with control animals. Feeding the 
      PUFA/EPAX diet restored T-cell proliferation in both groups of animals. We also 
      employed ionomycin, which at 50 nM opens calcium channels, and thapsigargin (TG), 
      which recruits [Ca(2+)]i from endoplasmic reticulum pool. We observed that 
      ionomycin-induced increases in [Ca(2+)]i in T-cells of diabetic mothers and obese 
      offspring were greater than in those of control rats. Furthermore, feeding 
      PUFA/EPAX diet diminished significantly the ionomycin-evoked rise in [Ca(2+)]i in 
      diabetic and obese animals. TG-induced increases in [Ca(2+)]i in T-cells of 
      diabetic pregnant rats and their obese offspring were greater than in those of 
      control rats. The feeding of the experimental diet significantly curtailed the 
      TG-evoked increases in [Ca(2+)]i in both diabetic and obese rats. DISCUSSION: 
      Together, these observations provide evidence that T-cell activation and T-cell 
      calcium signaling are altered during gestational diabetes and macrosomia. Hence, 
      dietary fish oils, particularly eicosapentaenoic acid and docosahexaenoic acid, 
      may restore these T-cell abnormalities.
FAU - Guermouche, Baya
AU  - Guermouche B
AD  - Department de Biologie, Faculte des Sciences, Universite de Tlemcen, Algeria.
FAU - Yessoufou, Akadiri
AU  - Yessoufou A
FAU - Soulimane, Nassima
AU  - Soulimane N
FAU - Merzouk, Hafida
AU  - Merzouk H
FAU - Moutairou, Kabirou
AU  - Moutairou K
FAU - Hichami, Aziz
AU  - Hichami A
FAU - Khan, Naim Akhtar
AU  - Khan NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obes Res
JT  - Obesity research
JID - 9305691
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 11028-71-0 (Concanavalin A)
RN  - 56092-81-0 (Ionomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Concanavalin A/pharmacology
MH  - Diabetes Mellitus, Experimental/immunology
MH  - Dietary Fats, Unsaturated/administration & dosage
MH  - Fatty Acids, Omega-3/*administration & dosage
MH  - Female
MH  - Fetal Macrosomia/etiology/*immunology
MH  - Gestational Age
MH  - Ionomycin/pharmacology
MH  - Lymphocyte Activation/drug effects
MH  - Obesity/etiology/*immunology
MH  - Pregnancy
MH  - Pregnancy in Diabetics/complications/*immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Spleen
MH  - T-Lymphocytes/*immunology/metabolism
MH  - Thapsigargin/pharmacology
EDAT- 2004/12/17 09:00
MHDA- 2005/03/18 09:00
CRDT- 2004/12/17 09:00
PHST- 2004/12/17 09:00 [pubmed]
PHST- 2005/03/18 09:00 [medline]
PHST- 2004/12/17 09:00 [entrez]
AID - 12/11/1744 [pii]
AID - 10.1038/oby.2004.216 [doi]
PST - ppublish
SO  - Obes Res. 2004 Nov;12(11):1744-53. doi: 10.1038/oby.2004.216.