PMID- 15603880
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 12
DP  - 2004 Dec
TI  - Discordant expression of HLA class II-associated co-chaperones and HLA-DRB 
      alleles in cultured fibroblast-like synoviocytes.
PG  - 1516-29
AB  - Human leukocyte antigen (HLA)-DR-positive synovial fibroblasts are frequently 
      observed in rheumatoid arthritis (RA) and may be implicated in the autoimmune 
      reaction because RA is associated with certain HLA-DRB1* alleles. The question of 
      whether components of the class II antigen presentation pathway and specific DRB 
      alleles are efficiently expressed by synovial fibroblasts is germane to this 
      hypothesis. To address this, cultured fibroblast-like synoviocytes (cFLS) were 
      analyzed for constitutive and interferon (IFN)-gamma-induced expression of 
      specific DRB alleles and class II-associated cochaperones. IFN-gamma induction of 
      invariant chain, DM, and DR molecules was observed in all cFLS, but expression of 
      specific DR allotypes was variable. Interestingly, DM-modulated epitopes on 
      RA-associated DR molecules were either absent or delayed, despite strong DM 
      expression and a paucity of major histocompatibility complex/class II-associated 
      invariant chain peptide complexes. Altered expression of specific 
      peptide-dependent epitopes on RA-associated HLA-DR molecules suggests differences 
      in antigen presentation by cFLS, which may have implications for the 
      immunopathogenesis of RA.
FAU - Spurrell, David R
AU  - Spurrell DR
AD  - Faculty of Medicine, Memorial University of Newfoundland, St. John's, 
      Newfoundland and Labrador, Canada.
FAU - Oldford, Sharon A
AU  - Oldford SA
FAU - Frost, Toby
AU  - Frost T
FAU - Larsen, Bodil
AU  - Larsen B
FAU - Codner, Dianne
AU  - Codner D
FAU - Edgecombe, Allison
AU  - Edgecombe A
FAU - Drover, Sheila
AU  - Drover S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (HLA-DO antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Recombinant Proteins)
RN  - 0 (invariant chain)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Antigen Presentation
MH  - Antigens, Differentiation, B-Lymphocyte/biosynthesis/genetics
MH  - Arthritis, Rheumatoid/*genetics/*immunology/pathology
MH  - Base Sequence
MH  - Cells, Cultured
MH  - DNA/genetics
MH  - Fibroblasts/immunology/pathology
MH  - Gene Expression
MH  - HLA-D Antigens/biosynthesis/*genetics
MH  - HLA-DR Antigens/biosynthesis/genetics
MH  - Histocompatibility Antigens Class II/biosynthesis/genetics
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Molecular Chaperones/biosynthesis/genetics
MH  - Recombinant Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/*immunology/pathology
EDAT- 2004/12/18 09:00
MHDA- 2005/04/01 09:00
CRDT- 2004/12/18 09:00
PHST- 2003/12/18 00:00 [received]
PHST- 2004/09/03 00:00 [revised]
PHST- 2004/09/09 00:00 [accepted]
PHST- 2004/12/18 09:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2004/12/18 09:00 [entrez]
AID - S0198-8859(04)00625-1 [pii]
AID - 10.1016/j.humimm.2004.09.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Dec;65(12):1516-29. doi: 10.1016/j.humimm.2004.09.005.