PMID- 15605362
OWN - NLM
STAT- MEDLINE
DCOM- 20050317
LR  - 20191210
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 105
IP  - 1
DP  - 2005 Feb 25
TI  - Comparison of molecular abnormalities in bronchial brushings and tumor touch 
      preparations.
PG  - 35-43
AB  - BACKGROUND: Preneoplastic lung lesions and early-stage lung carcinomas are 
      associated with molecular abnormalities. The authors performed a pilot study to 
      evaluate the use of DNA fluorescence in situ hybridization (FISH) probes to 
      ascertain whether these biomarkers can predict nonsmall cell lung carcinoma 
      (NSCLC). METHODS: Fourteen bronchial brushings ipsilateral to the tumor (BB/Ts), 
      tumor touch imprints, and touch imprints of the bronchus adjacent to the tumor 
      obtained from 15 patients with early-stage NSCLC were analyzed. The LAVysion 
      multicolor probe set consisting of probes to 5p15, 6, 7p12, and 8q2 and the 
      in-house probes 3p22.1 and 10q22 was used. Using the LAVysion multicolor probe 
      set, 25 epithelial cells were counted and considered positive if > 5 cells were 
      abnormal. Using 3p22.1 and 10q22, > or = 100 nuclei per slide were scored. The 
      results were tabulated as the percentage of cells with deletions compared with 
      the centromeric probes 3 and 10. Greater than 2% of the deletions were positive 
      for 3p22.1 and 10q22. Bronchial washings from patients without lung tumors were 
      used as controls. RESULTS: The BB/Ts were negative for malignant cells by 
      cytologic evaluation and the LAVysion probe set; however, the combined in-house 
      probes for 3p22.1 and 10q22 tested on BB/Ts predicted cancer in 100% of cancer 
      patients. FISH positivity in the lung cancers was 100% for 3p22.1 deletions, 79% 
      for 10q22 deletions, and 57% for LAVysion probes. When compared with the 
      bronchial epithelium, tumor cells showed a 3.7-fold excess of 3p22.1 deletions, a 
      2-fold excess of 10q22 deletions, and a 12.6-fold excess of abnormal cells. 
      CONCLUSIONS: The current study indicated that detection of molecular 
      abnormalities in bronchial epithelial cells via FISH was very useful in 
      identifying patients at high risk for developing lung carcinoma. The molecular 
      abnormalities identified in the BB/Ts were detected at elevated levels in the 
      tumor specimens.
CI  - 2004 American Cancer Society
FAU - Barkan, Guliz A
AU  - Barkan GA
AD  - Department of Pathology, The University of Texas M. D. Anderson Cancer Center, 
      Houston, Texas 77030, USA.
FAU - Caraway, Nancy P
AU  - Caraway NP
FAU - Jiang, Feng
AU  - Jiang F
FAU - Zaidi, Tanweer M
AU  - Zaidi TM
FAU - Fernandez, Ricardo
AU  - Fernandez R
FAU - Vaporcyin, Ara
AU  - Vaporcyin A
FAU - Morice, Rodolfo
AU  - Morice R
FAU - Zhou, Xian
AU  - Zhou X
FAU - Bekele, Benjamin Nebiyou
AU  - Bekele BN
FAU - Katz, Ruth L
AU  - Katz RL
LA  - eng
GR  - P50CA70907/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
EDAT- 2004/12/18 09:00
MHDA- 2005/03/18 09:00
CRDT- 2004/12/18 09:00
PHST- 2004/12/18 09:00 [pubmed]
PHST- 2005/03/18 09:00 [medline]
PHST- 2004/12/18 09:00 [entrez]
AID - 10.1002/cncr.20800 [doi]
PST - ppublish
SO  - Cancer. 2005 Feb 25;105(1):35-43. doi: 10.1002/cncr.20800.