PMID- 15606363
OWN - NLM
STAT- MEDLINE
DCOM- 20050822
LR  - 20131121
IS  - 0956-5507 (Print)
IS  - 0956-5507 (Linking)
VI  - 15
IP  - 6
DP  - 2004 Dec
TI  - Assessing HER-2 status in fresh frozen and archival cytological samples obtained 
      by fine needle aspiration cytology.
PG  - 311-4
AB  - Determination of HER-2 status is an essential prerequisite in considering a 
      patient's eligibility for anti-HER-2 therapy; few studies have focused on its 
      evaluation on cytological material. We present a new method of assessing HER-2 
      status on cytological samples, by fluorescence in situ hybridization (FISH) with 
      an automated detection system, using fresh frozen (FF) and May-Grunwald-Giemsa 
      (MGG) stained smears, and we evaluate the reliability of HER-2 determination on 
      fine needle aspiration cytology (FNAC). The pre-treatment protocol for FF smears 
      is easier and faster than for MGG stained slides. However, with the described 
      procedure, FISH is also feasible on archival MGG stained slides. We conclude that 
      with this method, cytological samples obtained by FNAC, either FF or MGG, are a 
      reliable option for assessing HER-2 status.
FAU - Tomas, A R
AU  - Tomas AR
AD  - Servico de Patologia Morfologica, Instituto Portugues de Oncologia Francisco 
      Gentil--CROL, S.A., Lisboa, Portugal. raqueltomas@netcabo.pt
FAU - Praca, M J
AU  - Praca MJ
FAU - Fonseca, R
AU  - Fonseca R
FAU - Andre, S
AU  - Andre S
FAU - Mendonca, E
AU  - Mendonca E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytopathology
JT  - Cytopathology : official journal of the British Society for Clinical Cytology
JID - 9010345
RN  - 0 (May-Grunwald Giemsa)
RN  - T42P99266K (Methylene Blue)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
SB  - IM
MH  - Biopsy, Fine-Needle
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Cryopreservation
MH  - Eosine Yellowish-(YS)
MH  - Female
MH  - Gene Amplification/genetics
MH  - Genes, erbB-2/*genetics
MH  - Histocytological Preparation Techniques
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Methylene Blue
MH  - Reproducibility of Results
MH  - Staining and Labeling
EDAT- 2004/12/21 09:00
MHDA- 2005/08/23 09:00
CRDT- 2004/12/21 09:00
PHST- 2004/12/21 09:00 [pubmed]
PHST- 2005/08/23 09:00 [medline]
PHST- 2004/12/21 09:00 [entrez]
AID - CYT210 [pii]
AID - 10.1111/j.1365-2303.2004.00210.x [doi]
PST - ppublish
SO  - Cytopathology. 2004 Dec;15(6):311-4. doi: 10.1111/j.1365-2303.2004.00210.x.