PMID- 15606547
OWN - NLM
STAT- MEDLINE
DCOM- 20050304
LR  - 20081121
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 128
IP  - 1
DP  - 2005 Jan
TI  - Increased interferon gamma production by peripheral blood mononuclear cells in 
      response to stimulation of overexpressed disease-specific 9-O-acetylated 
      sialoglycoconjugates in children suffering from acute lymphoblastic leukaemia.
PG  - 35-41
AB  - Disease-specific over-expression of 9-O-acetylated sialoglycoconjugates 
      (9-O-AcSGs) on peripheral blood mononuclear cells (PBMC) of children with acute 
      lymphoblastic leukaemia (ALL, PBMC(ALL)) has been demonstrated using a lectin, 
      Achatinin-H, with specificity towards 9-O-AcSAalpha2-6GalNAc. This study 
      investigated the contributory role of 9-O-AcSGs induced on PBMC(ALL). Stimulation 
      of PBMC(ALL) with Achatinin-H through 9-O-AcSGs led to a lymphoproliferative 
      response with a significantly increased interferon-gamma (IFN-gamma) production 
      when compared with unstimulated cells as demonstrated by enzyme-linked 
      immunosorbent assay and mRNA expression. Under identical conditions, PBMC(ALL) 
      ablated of O-acetylations did not respond to such stimulation. In summary, it may 
      be concluded that stimulation of over-expressed 9-O-AcSGs regulate signalling for 
      proliferation, leading to the release of IFN-gamma. Controlled expression of 
      these molecules may be exploited as potential targets for therapy, promising 
      beneficial effects to children with ALL.
FAU - Ghosh, Shyamasree
AU  - Ghosh S
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Kolkata, India.
FAU - Bandyopadhyay, Suman
AU  - Bandyopadhyay S
FAU - Pal, Santanu
AU  - Pal S
FAU - Das, Benubrata
AU  - Das B
FAU - Bhattacharya, Dilip K
AU  - Bhattacharya DK
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Glycoconjugates)
RN  - 0 (Lectins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sialic Acids)
RN  - 0 (achatinin(H))
RN  - 55717-54-9 (9-O-acetyl-N-acetylneuraminic acid)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Cell Proliferation
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Flow Cytometry
MH  - Glycoconjugates/genetics/*metabolism
MH  - Humans
MH  - Infant
MH  - Interferon-gamma/*immunology
MH  - Lectins/metabolism
MH  - Leukocytes, Mononuclear/*chemistry/immunology
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/*immunology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sialic Acids/genetics/*metabolism
MH  - Signal Transduction/physiology
MH  - Stimulation, Chemical
EDAT- 2004/12/21 09:00
MHDA- 2005/03/05 09:00
CRDT- 2004/12/21 09:00
PHST- 2004/12/21 09:00 [pubmed]
PHST- 2005/03/05 09:00 [medline]
PHST- 2004/12/21 09:00 [entrez]
AID - BJH5256 [pii]
AID - 10.1111/j.1365-2141.2004.05256.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 Jan;128(1):35-41. doi: 10.1111/j.1365-2141.2004.05256.x.