PMID- 15608336
OWN - NLM
STAT- MEDLINE
DCOM- 20051024
LR  - 20240109
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 17
IP  - 1
DP  - 2005 Jan
TI  - Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen 
      gene network of Arabidopsis.
PG  - 268-81
AB  - Reactive oxygen species (ROS), such as O2- and H2O2, play a key role in plant 
      metabolism, cellular signaling, and defense. In leaf cells, the chloroplast is 
      considered to be a focal point of ROS metabolism. It is a major producer of O2- 
      and H2O2 during photosynthesis, and it contains a large array of ROS-scavenging 
      mechanisms that have been extensively studied. By contrast, the function of the 
      cytosolic ROS-scavenging mechanisms of leaf cells is largely unknown. In this 
      study, we demonstrate that in the absence of the cytosolic H2O2-scavenging enzyme 
      ascorbate peroxidase 1 (APX1), the entire chloroplastic H2O2-scavenging system of 
      Arabidopsis thaliana collapses, H2O2 levels increase, and protein oxidation 
      occurs. We further identify specific proteins oxidized in APX1-deficient plants 
      and characterize the signaling events that ensue in knockout-Apx1 plants in 
      response to a moderate level of light stress. Using a dominant-negative approach, 
      we demonstrate that heat shock transcription factors play a central role in the 
      early sensing of H2O2 stress in plants. Using knockout plants for the NADPH 
      oxidase D protein (knockout-RbohD), we demonstrate that RbohD might be required 
      for ROS signal amplification during light stress. Our study points to a key role 
      for the cytosol in protecting the chloroplast during light stress and provides 
      evidence for cross-compartment protection of thylakoid and stromal/mitochondrial 
      APXs by cytosolic APX1.
FAU - Davletova, Sholpan
AU  - Davletova S
AD  - Department of Biochemistry and Molecular Biology, University of Nevada, Reno, 
      Nevada 89557, USA.
FAU - Rizhsky, Ludmila
AU  - Rizhsky L
FAU - Liang, Hongjian
AU  - Liang H
FAU - Shengqiang, Zhong
AU  - Shengqiang Z
FAU - Oliver, David J
AU  - Oliver DJ
FAU - Coutu, Jesse
AU  - Coutu J
FAU - Shulaev, Vladimir
AU  - Shulaev V
FAU - Schlauch, Karen
AU  - Schlauch K
FAU - Mittler, Ron
AU  - Mittler R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20041217
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Chloroplast Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.11 (APXS protein, Arabidopsis)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Arabidopsis/*enzymology/genetics
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Ascorbate Peroxidases
MH  - Chloroplast Proteins
MH  - Chloroplasts/*enzymology/genetics
MH  - Cytosol/*enzymology
MH  - Gene Expression Regulation, Plant/genetics
MH  - Hydrogen Peroxide/metabolism
MH  - Light
MH  - Mitochondria/enzymology/genetics
MH  - NADPH Oxidases/genetics/metabolism
MH  - Peroxidases/genetics/*metabolism
MH  - Photic Stimulation
MH  - Plant Leaves/*enzymology/genetics
MH  - Reactive Oxygen Species/*metabolism
MH  - Thylakoids/enzymology/genetics
PMC - PMC544504
EDAT- 2004/12/21 09:00
MHDA- 2005/10/26 09:00
PMCR- 2006/01/01
CRDT- 2004/12/21 09:00
PHST- 2004/12/21 09:00 [pubmed]
PHST- 2005/10/26 09:00 [medline]
PHST- 2004/12/21 09:00 [entrez]
PHST- 2006/01/01 00:00 [pmc-release]
AID - tpc.104.026971 [pii]
AID - 026971 [pii]
AID - 10.1105/tpc.104.026971 [doi]
PST - ppublish
SO  - Plant Cell. 2005 Jan;17(1):268-81. doi: 10.1105/tpc.104.026971. Epub 2004 Dec 17.