PMID- 15610609
OWN - NLM
STAT- MEDLINE
DCOM- 20050505
LR  - 20121115
IS  - 1043-0342 (Print)
IS  - 1043-0342 (Linking)
VI  - 15
IP  - 11
DP  - 2004 Nov
TI  - Selective transduction of malignant glioma by lentiviral vectors pseudotyped with 
      lymphocytic choriomeningitis virus glycoproteins.
PG  - 1091-100
AB  - Malignant gliomas are the most frequent primary brain tumors and have a dismal 
      prognosis due to their infiltrative growth. Gene therapy using viral vectors 
      represents an attractive alternative to conventional cancer therapies. In a 
      previous study, we established lentiviral vectors pseudotyped with lymphocytic 
      choriomeningitis virus (LCMV) glycoproteins (GPs) and demonstrated transduction 
      of human malignant glioma cells in culture. In the current approach, we compared 
      the transduction efficacy of LCMV-GP- and vesicular stomatitis virus glycoprotein 
      (VSV-G)-pseudotyped lentiviral vectors for malignant glioma cells and normal 
      brain cells in vitro and in vivo. LCMV-GP pseudotypes transduced almost 
      exclusively astrocytes, whereas VSV-G pseudotypes infected neurons as well as 
      astrocytes. LCMV-GP pseudotypes showed an efficient transduction of solid glioma 
      parts and specific transduction of infiltrating tumor cells. In contrast, 
      VSV-G-pseudotyped lentiviral vectors transduced only a few tumor cells in solid 
      tumor parts and infected mostly normal brain cells in infiltrating tumor areas. 
      In conclusion, lentiviral vectors pseudotyped with LCMV glycoproteins represent 
      an attractive option for gene therapy of malignant glioma.
FAU - Miletic, Hrvoje
AU  - Miletic H
AD  - Abteilung fur Neuropathologie, Universitat zu Koln, D-50931 Cologne, Germany. 
      h.miletic@uni-koeln.de
FAU - Fischer, Yvonne Heidemarie
AU  - Fischer YH
FAU - Neumann, Harald
AU  - Neumann H
FAU - Hans, Volkmar
AU  - Hans V
FAU - Stenzel, Werner
AU  - Stenzel W
FAU - Giroglou, Tsanan
AU  - Giroglou T
FAU - Hermann, Manuel
AU  - Hermann M
FAU - Deckert, Martina
AU  - Deckert M
FAU - Von Laer, Dorothee
AU  - Von Laer D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Glycoproteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (fluorescent protein 583)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Brain/embryology/metabolism/pathology
MH  - Brain Neoplasms/metabolism/*therapy
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors
MH  - Glioma/*genetics/metabolism/*pathology/*therapy
MH  - Glycoproteins/*genetics
MH  - Hippocampus/embryology/metabolism
MH  - Humans
MH  - Lentivirus/genetics
MH  - Luminescent Proteins/pharmacology
MH  - Lymphocytic choriomeningitis virus/*genetics
MH  - Microscopy, Fluorescence
MH  - Neurons/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Wistar
EDAT- 2004/12/22 09:00
MHDA- 2005/05/06 09:00
CRDT- 2004/12/22 09:00
PHST- 2004/12/22 09:00 [pubmed]
PHST- 2005/05/06 09:00 [medline]
PHST- 2004/12/22 09:00 [entrez]
AID - 10.1089/hum.2004.15.1091 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2004 Nov;15(11):1091-100. doi: 10.1089/hum.2004.15.1091.