PMID- 15610859
OWN - NLM
STAT- MEDLINE
DCOM- 20050510
LR  - 20061115
IS  - 1078-1439 (Print)
IS  - 1078-1439 (Linking)
VI  - 22
IP  - 6
DP  - 2004 Nov-Dec
TI  - Detection of C-erb B2 gene amplification in bilharzial associated bladder cancer 
      using fluorescence in situ hybridization.
PG  - 448-52
AB  - BACKGROUND: Gene amplifications are common events in different tumor types and 
      may confer diagnostic, prognostic, or therapeutic information for patient 
      management. Fluorescence in situ hybridization (FISH) represents a standard 
      methodologic approach for testing for this genetic alteration, as it is rapid, 
      reproducible and extremely reliable in detecting presence of C-erb-B2 gene 
      amplification for clinical utility. PATIENTS AND METHODS: In this study, FISH is 
      used in a series of archival human bilharzial bladder cancer specimens to 
      evaluate for the presence of cerbB-2 gene alterations in the most common 
      malignant tumor in bilharzial endemic areas, e.g., Egypt and some other 
      countries. The study included 40 cases, 30 males and 10 females. Their ages 
      ranged between 30 years and 76 years (median: 51 years). Twenty-one cases had 
      squamous cell carcinoma, 16 had transitional cell carcinoma, two had 
      adenocarcinoma, and one case had undifferentiated carcinoma. RESULTS: Thirteen 
      out of 40 tumor samples (32.5%) show evidence of true C-erb-B2 gene 
      amplification. Of the remaining samples, 24 (60%) show no gene amplification and 
      three (7.5%) fall into the borderline category with a ratio between one and two 
      C-erb-B2 genes/cell relative to chromosome 17 centromeres. No evidence of 
      chromosome 17 polysomy was found in any cases scored as single copy with the 
      C-erb-B2 probe. CONCLUSION: No significant association was found between gene 
      amplification and any of the tested clinicopathologic parameters or tumor 
      recurrence except for tumor grade where higher tumor grades tended to be 
      associated with more C-erb-B2 gene amplification (P = 0.01) thus reflecting more 
      tumor aggressiveness. So, the amplification of C-erb-B2 in bilharzial associated 
      bladder cancer is probably not independently related to clinical outcome of 
      patients.
FAU - Aly, Magdy Sayed
AU  - Aly MS
AD  - Biology Department, Faculty of Science, Cairo University (Beni-Suef Branch), 
      Cairo, Egypt.
FAU - Khaled, Hussein Mostafa
AU  - Khaled HM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Urol Oncol
JT  - Urologic oncology
JID - 9805460
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma/*genetics/parasitology
MH  - Female
MH  - Gene Amplification
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Schistosomiasis/*complications
MH  - Urinary Bladder Neoplasms/*genetics/parasitology
EDAT- 2004/12/22 09:00
MHDA- 2005/05/11 09:00
CRDT- 2004/12/22 09:00
PHST- 2003/09/03 00:00 [received]
PHST- 2004/07/07 00:00 [revised]
PHST- 2004/07/08 00:00 [accepted]
PHST- 2004/12/22 09:00 [pubmed]
PHST- 2005/05/11 09:00 [medline]
PHST- 2004/12/22 09:00 [entrez]
AID - S1078-1439(04)00175-9 [pii]
AID - 10.1016/j.urolonc.2004.07.016 [doi]
PST - ppublish
SO  - Urol Oncol. 2004 Nov-Dec;22(6):448-52. doi: 10.1016/j.urolonc.2004.07.016.