PMID- 15612018
OWN - NLM
STAT- MEDLINE
DCOM- 20050415
LR  - 20131121
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 482
IP  - 1
DP  - 2005 Jan 31
TI  - Epsilon-sarcoglycan immunoreactivity and mRNA expression in mouse brain.
PG  - 50-73
AB  - Myoclonus dystonia (M-D) is a hereditary movement disorder caused by a maternally 
      imprinted gene that is often associated with psychiatric symptoms. Most cases of 
      M-D are believed to result from mutations of the epsilon-sarcoglycan protein. The 
      neuroanatomical distribution of epsilon-sarcoglycan-like immunoreactivity in 
      mouse was investigated by using an antiserum against the epsilon-sarcoglycan 
      protein. The expression of epsilon-sarcoglycan mRNA was studied by a sensitive 
      fluorescence in situ hybridization (FISH) method. Immunohistochemistry and FISH 
      revealed a wide distribution of epsilon-sarcoglycan protein and mRNA throughout 
      the mouse brain. High expression levels of epsilon-sarcoglycan mRNA and 
      immunoreactivity were found in the mitral cell layer of the olfactory bulb, the 
      Purkinje cell layer in cerebellum, and the monoaminergic neurons in the mouse 
      midbrain. Immunohistochemistry revealed a similar distribution of 
      epsilon-sarcoglycan protein. Double-labeling FISH showed colocalization of 
      tyrosine hydroxylase and epsilon-sarcoglycan mRNAs within all the midbrain 
      dopaminergic (DAergic) cell groups. By combining FISH with fluorescence 
      immunohistochemistry, coexpression of epsilon-sarcoglycan mRNA and tryptophan 
      hydroxylase immunoreactivity was found in the serotonergic (5-HTergic) neurons 
      within the dorsal raphe nucleus. The distribution of epsilon-sarcoglycan in the 
      mouse brain suggests that the symptom complex of M-D may be related to the 
      effects of decreased epsilon-sarcoglycan activity on the development or function 
      of monoaminergic neurons.
CI  - 2004 Wiley-Liss, Inc.
FAU - Chan, Pokman
AU  - Chan P
AD  - Department of Neurology, Mount Sinai School of Medicine, New York, New York 
      10029, USA.
FAU - Gonzalez-Maeso, Javier
AU  - Gonzalez-Maeso J
FAU - Ruf, Frederique
AU  - Ruf F
FAU - Bishop, David F
AU  - Bishop DF
FAU - Hof, Patrick R
AU  - Hof PR
FAU - Sealfon, Stuart C
AU  - Sealfon SC
LA  - eng
GR  - R24 CA088309/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Biogenic Monoamines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sarcoglycans)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Biogenic Monoamines/metabolism
MH  - Brain/cytology/metabolism
MH  - Dopamine/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Mesencephalon/cytology/*metabolism
MH  - Mice
MH  - Neurons/metabolism
MH  - Olfactory Bulb/cytology/*metabolism
MH  - Purkinje Cells/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Sarcoglycans/genetics/*metabolism
MH  - Tissue Distribution
EDAT- 2004/12/22 09:00
MHDA- 2005/04/16 09:00
CRDT- 2004/12/22 09:00
PHST- 2004/12/22 09:00 [pubmed]
PHST- 2005/04/16 09:00 [medline]
PHST- 2004/12/22 09:00 [entrez]
AID - 10.1002/cne.20377 [doi]
PST - ppublish
SO  - J Comp Neurol. 2005 Jan 31;482(1):50-73. doi: 10.1002/cne.20377.