PMID- 15612783 OWN - NLM STAT- MEDLINE DCOM- 20050126 LR - 20061115 IS - 0021-8561 (Print) IS - 0021-8561 (Linking) VI - 52 IP - 26 DP - 2004 Dec 29 TI - Triacylglycerol composition of walnut (Juglans regia L.) cultivars: characterization by HPLC-ELSD and chemometrics. PG - 7964-9 AB - A total of 26 walnut (Juglans regia L.) samples from 9 cultivars (Arco, Franquette, Hartley, Lara, Marbot, Mayette, Mellanaise, Parisienne, and Rego) harvested in the 2001, 2002, and 2003 crop years and grown in two geographical origins (Braganca and Coimbra, Portugal) were evaluated with regard to their triacylglycerol composition. The methodology employed was reversed-phase high-performance liquid chromatography coupled to an evaporative light-scattering detector (RP-HPLC-ELSD) after extraction of the lipidic fraction of the nuts. Nine compounds were separated, identified, and quantified. All samples presented an identical qualitative profile composed by LLnLn, LLLn, LLL, OLLn, OLL, PLL, OOL, and PLO (P = palmitoyl; O = oleoyl; L = linoleoyl; Ln = linonenoyl). Trilinolein (LLL) was the major triglyceride, followed by dilinoeoyl-oleoyl-glycerol (OLL) and dilinoleoyl-linolenoyl-glycerol (LLLn), with mean values of 37.7, 18.5, and 18.4%, respectively. Significant differences in composition were found between cultivars, and these differences were also significant when cultivars were grouped by year of production, showing that besides genetic factors, the triacylglycerol composition can be strongly influenced by environmental factors. FAU - Amaral, Joana S AU - Amaral JS AD - REQUIMTE, Servicos de Farmacognosia and Bromatologia, Faculdade de Farmacia, Universidade do Porto, R. Anibal Cunha 164, 4050-047 Porto, Portugal. jamaral@ipb.pt FAU - Cunha, Sara C AU - Cunha SC FAU - Alves, M Rui AU - Alves MR FAU - Pereira, Jose A AU - Pereira JA FAU - Seabra, Rosa M AU - Seabra RM FAU - Oliveira, Beatriz P P AU - Oliveira BP LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Plant Oils) RN - 0 (Triglycerides) SB - IM MH - Chromatography, High Pressure Liquid/*methods MH - Juglans/*chemistry MH - Plant Oils/chemistry MH - Triglycerides/*analysis EDAT- 2004/12/23 09:00 MHDA- 2005/01/27 09:00 CRDT- 2004/12/23 09:00 PHST- 2004/12/23 09:00 [pubmed] PHST- 2005/01/27 09:00 [medline] PHST- 2004/12/23 09:00 [entrez] AID - 10.1021/jf048918n [doi] PST - ppublish SO - J Agric Food Chem. 2004 Dec 29;52(26):7964-9. doi: 10.1021/jf048918n.