PMID- 15614612
OWN - NLM
STAT- MEDLINE
DCOM- 20050323
LR  - 20181113
IS  - 1567-2379 (Print)
IS  - 1567-2379 (Linking)
VI  - 35
IP  - 6
DP  - 2004 Aug
TI  - Novel bright field molecular morphology methods for detection of HER2 gene 
      amplification.
PG  - 589-94
AB  - Profiling the amplification and over-expression of the HER2 gene is a key 
      component for defining the prognosis and management of invasive breast carcinoma. 
      Clinical laboratory testing for HER2 gene amplification and over expression has 
      been complicated by an unacceptably high rate of false positive 
      immunohistochemistry (IHC) results, poor reproducibility for the '2+' category of 
      IHC scoring, and reluctant acceptance of alternative testing by fluorescence in 
      situ hybridization (FISH) by the diagnostic pathology community. Novel 
      chromogenic in situ hybridization (CISH) assays have been developed that utilize 
      bright field microscopy and a conventional light microscope for interpretation, 
      but the analytical sensitivity of first generation CISH systems has been 
      problematic. Novel second generation in situ hybridization detection methods 
      based upon polymerized lg detection chemistry, autometallography or enzyme 
      metallography, have been developed that routinely detect endogenous HER2 signals 
      in normal cells (on slide hybridization control) and HER2 signals in both 
      non-amplified and amplified patterns of HER2 genomic signatures. By combining the 
      strength of polymerized peroxidase-labeled antibodies and metallography for gene 
      amplification, with the detection of expression of HER2 encoded protein by IHC on 
      the same slide, both HER2 gene amplification and protein over-expression can be 
      simultaneously evaluated on a cell-by-cell basis in each microscopic field of 
      carcinoma.
FAU - Tubbs, Raymond
AU  - Tubbs R
AD  - Department of Anatomic, The Cleveland Clinic Foundation and The Cleveland Clinic 
      Lerner College of Medicine, Cleveland, Ohio 44195, USA.
FAU - Pettay, James
AU  - Pettay J
FAU - Hicks, David
AU  - Hicks D
FAU - Skacel, Marek
AU  - Skacel M
FAU - Powell, Richard
AU  - Powell R
FAU - Grogan, Tom
AU  - Grogan T
FAU - Hainfeld, James
AU  - Hainfeld J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Metals)
SB  - IM
MH  - Breast Neoplasms/genetics
MH  - Female
MH  - *Gene Amplification
MH  - Genes, Reporter
MH  - *Genes, erbB-2
MH  - Histocytochemistry/methods
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Metals/chemistry
EDAT- 2004/12/23 09:00
MHDA- 2005/03/24 09:00
CRDT- 2004/12/23 09:00
PHST- 2003/12/04 00:00 [received]
PHST- 2004/03/11 00:00 [revised]
PHST- 2004/12/23 09:00 [pubmed]
PHST- 2005/03/24 09:00 [medline]
PHST- 2004/12/23 09:00 [entrez]
AID - 10.1007/s10735-004-2191-9 [doi]
PST - ppublish
SO  - J Mol Histol. 2004 Aug;35(6):589-94. doi: 10.1007/s10735-004-2191-9.