PMID- 15615775
OWN - NLM
STAT- MEDLINE
DCOM- 20050714
LR  - 20091119
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 118
IP  - Pt 2
DP  - 2005 Jan 15
TI  - Role of CBP in regulating HIF-1-mediated activation of transcription.
PG  - 301-11
AB  - The hypoxia-inducible factor-1 (HIF-1) is a key regulator of oxygen homeostasis 
      in the cell. We have previously shown that HIF-1alpha and the transcriptional 
      coactivator CBP colocalize in accumulation foci within the nucleus of hypoxic 
      cells. In our further exploration of the hypoxia-dependent regulation of 
      HIF-1alpha function by transcriptional coactivators we observed that coexpression 
      of SRC-1 (another important coactivator of the hypoxia response) and HIF-1alpha 
      did not change the individual characteristic nuclear distribution patterns. 
      Colocalization of both these proteins proved to be mediated by CBP. Biochemical 
      assays showed that depletion of CBP from cell extracts abrogated interaction 
      between SRC-1 and HIF-1alpha. Thus, in contrast to the current model for the 
      assembly of complexes between nuclear hormone receptors and coactivators, the 
      present data suggest that it is CBP that recruits SRC-1 to HIF-1alpha in hypoxic 
      cells. We also observed that CBP, HIF-1alpha/Arnt and HIF-1alpha/CBP accumulation 
      foci partially overlap with the hyperphosphorylated form of RNA polymerase II, 
      and that CBP had a stabilizing effect on the formation of the complex between 
      HIF-1alpha and its DNA-binding partner, Arnt. In conclusion, CBP plays an 
      important role as a mediator of HIF-1alpha/Arnt/CBP/SRC-1 complex formation, 
      coordinating the temporally and hierarchically regulated intranuclear traffic of 
      HIF-1alpha and associated cofactors in signal transduction in hypoxic cells.
FAU - Ruas, Jorge L
AU  - Ruas JL
AD  - Department of Cell and Molecular Biology, Karolinska Institute, 171 77 Stockholm, 
      Sweden.
FAU - Poellinger, Lorenz
AU  - Poellinger L
FAU - Pereira, Teresa
AU  - Pereira T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041222
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (ARNT protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Active Transport, Cell Nucleus/physiology
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator
MH  - Cell Hypoxia/physiology
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Luminescent Proteins/genetics
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - Point Mutation
MH  - Receptors, Aryl Hydrocarbon/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcriptional Activation/*physiology
EDAT- 2004/12/24 09:00
MHDA- 2005/07/15 09:00
CRDT- 2004/12/24 09:00
PHST- 2004/12/24 09:00 [pubmed]
PHST- 2005/07/15 09:00 [medline]
PHST- 2004/12/24 09:00 [entrez]
AID - jcs.01617 [pii]
AID - 10.1242/jcs.01617 [doi]
PST - ppublish
SO  - J Cell Sci. 2005 Jan 15;118(Pt 2):301-11. doi: 10.1242/jcs.01617. Epub 2004 Dec 
      22.