PMID- 15615886
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20131121
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 85
IP  - 1
DP  - 2005 May
TI  - Genotoxic effects on spermatozoa of carbaryl-exposed workers.
PG  - 615-23
AB  - Carbaryl, one of the most important insecticides, is widely produced and used. To 
      explore carbaryl-induced genotoxic effects of spermatozoa, particularly DNA 
      damage and chromosome aberrations (CA), we first examined conventional semen 
      parameters, the progression and motion parameters of the spermatozoa among 16 
      carbaryl-exposed workers and 30 internal and external control individuals. Sperm 
      DNA damage represented as positive percentage of DNA fragmentation was detected 
      by a modified terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick 
      end-labeling (TUNEL) assay. Then numerical CA of chromosome X, Y, and 18 were 
      investigated by multicolor fluorescence in situ hybridization (FISH). The results 
      showed significant differences in the percentage of sperm abnormality between 
      carbaryl-exposed group and the external control group (p = 0.008). Mean (+/-SD) 
      percentage of spermatozoa with fragmented DNA in carbaryl-exposed group (21.04 
      +/- 8.88%) was significantly higher than those in the internal (13.36 +/- 12.17%) 
      and external control groups (13.92 +/- 7.15%), respectively (p = 0.035 and p = 
      0.030). Using FISH, we observed the frequency of sperm sex chromosome disomy was 
      0.661 +/- 0.238% in the exposed group, which was significantly higher than that 
      in the external control group (0.386 +/- 0.140%) (p = 0.001), and the 
      carbaryl-exposed group (0.276 +/- 0.126%) had an elevated chromosome 18 disomy 
      compared with the internal (0.195 +/- 0.094%) and external control groups (0.124 
      +/- 0.068%), respectively (p < 0.05 and p < 0.01). In addition, carbaryl-exposed 
      donors had significantly higher sperm nullisomic frequencies of sex chromosomes 
      and chromosome 18 than the external controls (p < 0.01) but not the internal 
      controls. In summary, the frequencies of aneuploidy and numerical CA showed 
      significant differences between exposed group and control groups (p < 0.05 and/or 
      p < 0.01). Moreover, positive correlations were found between sex chromosome 
      disomy, aneuploidy rate, and morphologic abnormalities in spermatozoa of all 
      donors (r = 0.564 and r = 0.555, p < 0.01). Our findings suggested that carbaryl 
      might induce morphologic abnormalities and genotoxic defects of spermatozoa among 
      exposed workers by causing DNA fragmentation and numerical CA in spermatogenesis 
      as a potential genotoxicant. The evidence also indicated that the 
      spermatotoxicity induced by carbaryl exposure might be related to adverse 
      reproductive outcomes.
FAU - Xia, Yankai
AU  - Xia Y
AD  - The Key Laboratory of Reproductive Medicine of Jiangsu Province, Institute of 
      Toxicology, Nanjing Medical University, Nanjing 210029, China.
FAU - Cheng, Senping
AU  - Cheng S
FAU - Bian, Qian
AU  - Bian Q
FAU - Xu, Lichun
AU  - Xu L
FAU - Collins, Michael D
AU  - Collins MD
FAU - Chang, Hebron C
AU  - Chang HC
FAU - Song, Lin
AU  - Song L
FAU - Liu, Jiayin
AU  - Liu J
FAU - Wang, Shoulin
AU  - Wang S
FAU - Wang, Xinru
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041222
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Mutagens)
RN  - R890C8J3N1 (Carbaryl)
SB  - IM
MH  - Adult
MH  - Agriculture
MH  - Carbaryl/*adverse effects
MH  - Chromosome Aberrations/*chemically induced
MH  - Chromosomes, Human, Pair 18/drug effects
MH  - Chromosomes, Human, X/drug effects
MH  - Chromosomes, Human, Y/drug effects
MH  - DNA Fragmentation/drug effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Middle Aged
MH  - Mutagens/*adverse effects
MH  - Occupational Exposure/*adverse effects
MH  - Sperm Count
MH  - Sperm Motility/*drug effects
MH  - Spermatozoa/*drug effects/metabolism/ultrastructure
EDAT- 2004/12/24 09:00
MHDA- 2005/08/02 09:00
CRDT- 2004/12/24 09:00
PHST- 2004/12/24 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2004/12/24 09:00 [entrez]
AID - kfi066 [pii]
AID - 10.1093/toxsci/kfi066 [doi]
PST - ppublish
SO  - Toxicol Sci. 2005 May;85(1):615-23. doi: 10.1093/toxsci/kfi066. Epub 2004 Dec 22.