PMID- 15615902
OWN - NLM
STAT- MEDLINE
DCOM- 20050412
LR  - 20220309
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 129
IP  - 1
DP  - 2005 Jan
TI  - Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the 
      tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during 
      tubal pregnancy.
PG  - 103-13
AB  - Matrix metalloproteinases (MMPs) are responsible for extracellular matrix (ECM) 
      degradation, and their functions are regulated by tissue inhibitors of MMPs 
      (TIMPs). The evidence for the roles of MMPs and TIMPs in implantation and 
      placentation has remained insufficient in humans, especially during the early 
      stages. Tubal pregnancy has some similarities to normal intrauterine pregnancy 
      and therefore may provide a unique model for implantation studies. In the present 
      study, the expression of MMP-2, -9 and -14, and TIMP-1, -2 and -3 at the 
      feto-maternal interface during tubal pregnancy was examined by 
      immunohistochemistry and in situ hybridization. We found that MMP-9 and TIMP-1, 
      -2 and -3 are produced by all types of extravillous cytotrophoblast (EVCT) cells, 
      while MMP-2 and -14 mainly exist in distal column cytotrophoblast (CCT) cells and 
      invasive EVCT cells. Meanwhile, the intensity of MMP-14 and TIMP-1 and -2 
      increased along the invasive pathway toward maternal interstitium. In addition, 
      MMP-2, -9 and -14 and TIMP-1, -2 and -3 were all detected in the villous CT (VCT) 
      cells. Furthermore, both the mRNA level and immunoreactivity of MMP-9, TIMP-1 and 
      -3 increased, while those of TIMP-2 decreased concurrent with the progression of 
      pregnancy during weeks 3-9. The unique expression pattern of various MMPs and 
      TIMPs at the feto-maternal interface suggests that they may have roles in 
      regulating the controlled invasion of trophoblasts during implantation and 
      placentation. Meanwhile, the study provides a better understanding of the 
      mechanisms involved in cellular events during human pregnancy, especially at the 
      initiation stage of implantation.
FAU - Bai, S X
AU  - Bai SX
AD  - State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese 
      Academy of Sciences, Beijing 100080, China.
FAU - Wang, Y L
AU  - Wang YL
FAU - Qin, L
AU  - Qin L
FAU - Xiao, Z J
AU  - Xiao ZJ
FAU - Herva, R
AU  - Herva R
FAU - Piao, Y S
AU  - Piao YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases, Membrane-Associated)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - *Embryo Implantation
MH  - Fallopian Tubes/*enzymology
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 2/analysis
MH  - Matrix Metalloproteinase 9/analysis
MH  - Matrix Metalloproteinases/*analysis
MH  - Matrix Metalloproteinases, Membrane-Associated
MH  - Metalloendopeptidases/analysis
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Pregnancy, Tubal/*enzymology
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis
MH  - Tissue Inhibitor of Metalloproteinase-2/analysis
MH  - Tissue Inhibitor of Metalloproteinase-3/analysis
MH  - Tissue Inhibitor of Metalloproteinases/*analysis
EDAT- 2004/12/24 09:00
MHDA- 2005/04/13 09:00
CRDT- 2004/12/24 09:00
PHST- 2004/12/24 09:00 [pubmed]
PHST- 2005/04/13 09:00 [medline]
PHST- 2004/12/24 09:00 [entrez]
AID - 129/1/103 [pii]
AID - 10.1530/rep.1.00283 [doi]
PST - ppublish
SO  - Reproduction. 2005 Jan;129(1):103-13. doi: 10.1530/rep.1.00283.