PMID- 15616822
OWN - NLM
STAT- MEDLINE
DCOM- 20050707
LR  - 20181113
IS  - 0032-0935 (Print)
IS  - 0032-0935 (Linking)
VI  - 221
IP  - 2
DP  - 2005 May
TI  - Agropyron elongatum chromatin localization on the wheat chromosomes in an 
      introgression line.
PG  - 277-86
AB  - The introgressed small-chromosome segment of Agropyron elongatum (Host.) Neviski 
      (Thinopyrum ponticum Podp.) in F5 line II-1-3 of somatic hybrid between common 
      wheat (Triticum aestivum L.) and A. elongatum was localized by sequential 
      fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) 
      and karyotype data. Karyotype analysis offered basic data of arm ratios and 
      relative lengths of 21 pairs of chromosomes in parent wheat Jinan177 and hybrid 
      II-1-3. Using special high repetitive sequences pSc119.2 and pAs1 for FISH, the 
      entire B- and D-genome chromosomes were detected. The FISH pattern of hybrid 
      II-1-3 was the same as that of parent wheat. GISH using whole genomic DNA from A. 
      elongatum as probe determined the alien chromatin. Sequential GISH and FISH, in 
      combination with some of the karyotype data, localized the small chromosome 
      segments of A. elongatum on the specific sites of wheat chromosomes 2AL, 1BL, 
      5BS, 1DL, 2DL and 6DS. FISH with probe OPF-03(1296) from randomly amplified 
      polymorphic DNA (RAPD) detected E-genome chromatin of A. elongatum, which existed 
      in all of the small chromosome segments introgressed. Microsatellite primers 
      characteristic for the chromosome arms above were used to check the localization 
      and reveal the genetic identity. These methods are complementary and provide 
      comprehensive information about the genomic constitution of the hybrid. The 
      relationship between hybrid traits and alien chromatin was discussed.
FAU - Wang, Jing
AU  - Wang J
AD  - School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of 
      China. xiagm@sdu.edu.cn
FAU - Xiang, Fengning
AU  - Xiang F
FAU - Xia, Guangmin
AU  - Xia G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041223
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Chromatin)
SB  - IM
MH  - Agropyron/*genetics
MH  - Chromatin/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Plant/ultrastructure
MH  - Crosses, Genetic
MH  - Cytogenetic Analysis
MH  - In Situ Hybridization
MH  - Karyotyping
MH  - Triticum/*genetics
EDAT- 2004/12/24 09:00
MHDA- 2005/07/08 09:00
CRDT- 2004/12/24 09:00
PHST- 2004/10/27 00:00 [received]
PHST- 2004/11/01 00:00 [accepted]
PHST- 2004/12/24 09:00 [pubmed]
PHST- 2005/07/08 09:00 [medline]
PHST- 2004/12/24 09:00 [entrez]
AID - 10.1007/s00425-004-1443-y [doi]
PST - ppublish
SO  - Planta. 2005 May;221(2):277-86. doi: 10.1007/s00425-004-1443-y. Epub 2004 Dec 23.