PMID- 15620456
OWN - NLM
STAT- MEDLINE
DCOM- 20051011
LR  - 20231213
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 1
DP  - 2005 Jan
TI  - HLA-E surface expression is independent of the availability of HLA class I signal 
      sequence-derived peptides in human tumor cell lines.
PG  - 1-12
AB  - Human leukocyte antigen (HLA)-E is a nonclassic HLA class I molecule whose 
      expression at the cell surface of tumor cells might allow them to escape T- and 
      natural killer (NK)-cell immune surveillance. In this study, we analyzed HLA-E 
      expression in a panel of human HLA-typed tumor cell lines of different histotypes 
      by flow cytometry with anti-HLA-E monoclonal antibodies and by reverse 
      transcriptase-polymerase chain reaction. Although specific HLA-E transcripts were 
      detected in all cell lines, except in HELA, surface expression was detected at 
      different intensities on seven (23%) of 30 cell lines with higher frequency and 
      intensity among osteosarcoma cell lines. HLA-E-positive tumor cell lines mainly 
      expressed the HLA-A*02 class I allele. Some tumor cell lines demonstrating HLA 
      class I A* or Cw* alleles, which we expected to allow HLA-E surface expression on 
      the basis of reported data on lymphoid cells, instead were HLA-E negative. All 
      tumor cell lines were either tapasin and TAP-1 positive by flow cytometry, except 
      two osteosarcoma cell lines, a finding that suggests an intact assembly machinery 
      for peptide loading. We conclude that the concomitant presence of the appropriate 
      HLA class I alleles with leader sequence-derived peptides and HLA-E heavy chain 
      may not be sufficient to allow HLA-E surface expression in tumor cell lines as 
      opposed to lymphoid cells.
FAU - Palmisano, Giulio Lelio
AU  - Palmisano GL
AD  - Department of Biology, University of Genova, Italy.
FAU - Contardi, Elisabetta
AU  - Contardi E
FAU - Morabito, Anna
AU  - Morabito A
FAU - Gargaglione, Vittoria
AU  - Gargaglione V
FAU - Ferrara, Giovanni Battista
AU  - Ferrara GB
FAU - Pistillo, Maria Pia
AU  - Pistillo MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antiporters)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (TAP1 protein, human)
RN  - 0 (tapasin)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/biosynthesis/immunology
MH  - *Alleles
MH  - Antiporters/*immunology
MH  - Caco-2 Cells
MH  - Cell Membrane/*immunology/metabolism
MH  - Flow Cytometry
MH  - HLA Antigens/biosynthesis/*immunology
MH  - HT29 Cells
MH  - HeLa Cells
MH  - Histocompatibility Antigens Class I/biosynthesis/*immunology
MH  - Humans
MH  - Immunoglobulins/*immunology
MH  - Membrane Transport Proteins
MH  - *Protein Sorting Signals/genetics
MH  - HLA-E Antigens
EDAT- 2004/12/29 09:00
MHDA- 2005/10/12 09:00
CRDT- 2004/12/29 09:00
PHST- 2004/07/15 00:00 [received]
PHST- 2004/10/08 00:00 [revised]
PHST- 2004/10/14 00:00 [accepted]
PHST- 2004/12/29 09:00 [pubmed]
PHST- 2005/10/12 09:00 [medline]
PHST- 2004/12/29 09:00 [entrez]
AID - S0198-8859(04)00650-0 [pii]
AID - 10.1016/j.humimm.2004.10.006 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 Jan;66(1):1-12. doi: 10.1016/j.humimm.2004.10.006.