PMID- 15620464
OWN - NLM
STAT- MEDLINE
DCOM- 20051011
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 1
DP  - 2005 Jan
TI  - Lack of the appropriate natural killer cell inhibitory receptors in women with 
      spontaneous abortion.
PG  - 65-71
AB  - Previous studies have revealed that women with unexplained recurrent spontaneous 
      abortions have a limited repertoire of inhibitory KI receptors (inhKIRs) and that 
      the inhKIRs they possess do not have specificity for the human leukocyte antigen 
      (HLA)-Cw molecules that would be expressed on trophoblast. We sought to confirm 
      these findings by direct definition of maternal inhKIR and trophoblastic HLA-Cw 
      allotypes on the placental material of spontaneously missed pregnancies. The 
      study included 30 women undergoing vacuum uterine curettage for first-trimester 
      missed pregnancy (group A; n = 15) or for elective termination of normal 
      pregnancy (group C, n = 15). DNA extracted from isolated decidual and 
      trophoblastic cells was used for molecular detection of maternal inhKIRs (2DL1, 
      2DL2, 2DL3) and fetal HLA-Cw alleles, respectively. The results revealed that in 
      the group of women who experienced abortion, 60% did not have the full repertoire 
      of three inhKIRs (group A vs group C; p = 0.006); that in five of 15 patients 
      (none in the controls), no epitope matching existed between maternal inhKIRs and 
      trophoblastic HLA-Cw alleles (group A vs group C; p = 0.01); and that more cases 
      were found with limited epitope matching (less than three inhKIRs with 
      specificity for fetal HLA-Cw alleles). The results provide additional evidence 
      that in some cases of spontaneous abortions, the women lack the appropriate 
      inhKIRs to interact with the HLA-Cw molecules on trophoblasts and to deliver 
      signals to inhibit natural killer cell activation and protect the embryo.
FAU - Varla-Leftherioti, M
AU  - Varla-Leftherioti M
AD  - Department of Immunobiology, Helena Venizelou Maternity Hospital, 11521 Athens, 
      Greece. gr1bmdr@hol.gr
FAU - Spyropoulou-Vlachou, M
AU  - Spyropoulou-Vlachou M
FAU - Keramitsoglou, T
AU  - Keramitsoglou T
FAU - Papadimitropoulos, M
AU  - Papadimitropoulos M
FAU - Tsekoura, C
AU  - Tsekoura C
FAU - Graphou, O
AU  - Graphou O
FAU - Papadopoulou, C
AU  - Papadopoulou C
FAU - Gerondi, M
AU  - Gerondi M
FAU - Stavropoulos-Giokas, C
AU  - Stavropoulos-Giokas C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-C*12:03 antigen)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Abortion, Spontaneous/*immunology
MH  - Decidua/immunology
MH  - Female
MH  - HLA-C Antigens/*immunology
MH  - Humans
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Placenta/immunology
MH  - Pregnancy
MH  - Receptors, Immunologic/*immunology
MH  - Trophoblasts/*immunology
EDAT- 2004/12/29 09:00
MHDA- 2005/10/12 09:00
CRDT- 2004/12/29 09:00
PHST- 2004/06/14 00:00 [received]
PHST- 2004/10/07 00:00 [revised]
PHST- 2004/10/14 00:00 [accepted]
PHST- 2004/12/29 09:00 [pubmed]
PHST- 2005/10/12 09:00 [medline]
PHST- 2004/12/29 09:00 [entrez]
AID - S0198-8859(04)00649-4 [pii]
AID - 10.1016/j.humimm.2004.10.005 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 Jan;66(1):65-71. doi: 10.1016/j.humimm.2004.10.005.