PMID- 15621247
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20220409
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 26
IP  - 17
DP  - 2005 Jun
TI  - 3D microenvironment as essential element for osteoinduction by biomaterials.
PG  - 3565-75
AB  - In order to unravel the mechanism of osteoinduction by biomaterials, in this 
      study we investigated the influence of the specific surface area on 
      osteoinductive properties of two types of calcium phosphate ceramics. Different 
      surface areas of the ceramics were obtained by varying their sintering 
      temperatures. Hydroxyapatite (HA) ceramic was sintered at 1150 and 1250 degrees 
      C. Biphasic calcium phosphate (BCP) ceramic, consisting of HA and beta-tricalcium 
      phosphate (beta-TCP), was sintered at 1100, 1150 and 1200 degrees C. Changes in 
      sintering temperature did not influence the chemistry of the ceramics; HA 
      remained pure after sintering at different temperatures and the weight ratio of 
      HA and beta-TCP in the BCP was independent of the temperature as well. Similarly, 
      macroporosity of the ceramics was unaffected by the changes of the sintering 
      temperature. However, microporosity (pore diameter <10 microm) significantly 
      decreased with increasing sintering temperature. In addition to the decrease of 
      the microporosity, the crystal size increased with increasing sintering 
      temperature. These two effects resulted in a significant decrease of the specific 
      surface area of the ceramics with increasing sintering temperatures. Samples of 
      HA1150, HA1250, BCP1100, BCP1150 and BCP1200 were implanted in the back muscles 
      of Dutch milk goats and harvested at 6 and 12 weeks post implantation. After 
      explantation, histomorphometrical analysis was performed on all implants. All 
      implanted materials except HA1250 induced bone. However, large variations in the 
      amounts of induced bone were observed between different materials and between 
      individual animals. Histomorphometrical results showed that the presence of 
      micropores within macropore walls is necessary to make a material osteoinductive. 
      We postulate that introduction of microporosity within macropores, and consequent 
      increase of the specific surface area, affects the interface dynamics of the 
      ceramic in such a way that relevant cells are triggered to differentiate into the 
      osteogenic lineage.
FAU - Habibovic, Pamela
AU  - Habibovic P
AD  - Institute for Biomedical Technology, Twente University, Department Bilthoven, 
      Professor Bronkhorstlaan 10-D, Bilthoven, 3723 MB, The Netherlands.
FAU - Yuan, Huipin
AU  - Yuan H
FAU - van der Valk, Chantal M
AU  - van der Valk CM
FAU - Meijer, Gert
AU  - Meijer G
FAU - van Blitterswijk, Clemens A
AU  - van Blitterswijk CA
FAU - de Groot, Klaas
AU  - de Groot K
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 91D9GV0Z28 (Durapatite)
RN  - L11K75P92J (calcium phosphate, dibasic, anhydrous)
SB  - IM
MH  - Animals
MH  - Bone Substitutes/*chemistry
MH  - Bone and Bones/*pathology
MH  - Calcium Phosphates/*chemistry
MH  - Crystallization/methods
MH  - Durapatite/*chemistry
MH  - Female
MH  - Goats
MH  - Implants, Experimental
MH  - Materials Testing
MH  - Osseointegration/*physiology
MH  - Osteogenesis/*physiology
MH  - Porosity
MH  - Surface Properties
MH  - Temperature
MH  - Tissue Engineering/methods
EDAT- 2004/12/29 09:00
MHDA- 2005/06/21 09:00
CRDT- 2004/12/29 09:00
PHST- 2004/07/14 00:00 [received]
PHST- 2004/09/22 00:00 [accepted]
PHST- 2004/12/29 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2004/12/29 09:00 [entrez]
AID - S0142-9612(04)00883-X [pii]
AID - 10.1016/j.biomaterials.2004.09.056 [doi]
PST - ppublish
SO  - Biomaterials. 2005 Jun;26(17):3565-75. doi: 10.1016/j.biomaterials.2004.09.056.