PMID- 15622626
OWN - NLM
STAT- MEDLINE
DCOM- 20100421
LR  - 20120625
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 18
IP  - 6
DP  - 1997 Jun
TI  - [Detection of bcr gene rearrangement in chronic myeloid leukemia by fluorescence 
      in situ hybridization].
PG  - 308-10
AB  - OBJECTIVE: To detect bcr gene rearrangement in chronic myeloid leukemia (CML). 
      METHODS: Bcr rearrangement was detected by fluorescence in situ hybridization 
      (FISH), using 765E3, a yeast artificial chromosome (YAC)-derived probe flanking 
      the breakpoint within bcr gene. RESULTS: Nine patients with CML were examined, 
      bcr gene rearrangement was revealed in 5 cases in chronic phase, 2 in blastic 
      phase, and one after interferon-alpha therapy. The karyotype of 1 case after 
      autologous bone marrow transplantation (ABMT) was chimera with normal and bcr 
      gene rearrangement chromosomes. CONCLUSION: YAC765E3 is a useful probe for 
      detecting bcr gene rearrangement. FISH technique is likely an important tool for 
      monitoring of treatment and revealing minimal residual disease in CML.
FAU - Liu, M
AU  - Liu M
AD  - Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical 
      University, Shanghai 200025.
FAU - Liu, X
AU  - Liu X
FAU - Niu, C
AU  - Niu C
FAU - He, K
AU  - He K
FAU - Su, X
AU  - Su X
FAU - Cao, Q
AU  - Cao Q
FAU - Chen, L
AU  - Chen L
FAU - Chen, S
AU  - Chen S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - EC 2.7.11.1 (BCR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chronic Disease
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - Proto-Oncogene Proteins c-bcr/*genetics
EDAT- 1997/06/01 00:00
MHDA- 2010/04/22 06:00
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 2010/04/22 06:00 [medline]
PHST- 1997/06/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 1997 Jun;18(6):308-10.