PMID- 15625703
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20061115
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 70
IP  - 3
DP  - 2005 Mar
TI  - Identification of differentially expressed genes in murine embryos at the 
      blastocyst stage using annealing control primer system.
PG  - 278-87
AB  - The identification of embryo-specific genes would provide insights into early 
      embryonic development. However, the current methods employed to identify the 
      genes that are expressed at a specific developmental stage are labor intensive 
      and suffer from high rates of false positives. Here we employed a new and 
      accurate reverse transcription-polymerase chain reaction (RT-PCR) method that 
      involves annealing control primers (ACPs) to identify the genes that are 
      specifically or prominently expressed in mouse blastocysts compared to 4-cell 
      stage embryos. Using 120 ACPs, we identified and sequenced 74 of these 
      differentially expressed genes (DEGs). Basic Local Alignment Search Tool (BLAST) 
      searches revealed that 53 were known genes, 9 encoded ribosomal proteins, and 12 
      were unknown genes. Of the known genes, 14 were selected and further 
      characterized using real-time quantitative PCR to assess their stage-specific 
      expression in mouse embryos. This analysis suggests that the ACP system is a very 
      good method for the identification of stage-specific genes in small numbers of 
      mouse embryos. Further analysis of the differentially expressed blastocyst genes 
      we have identified will provide insights into the molecular basis of 
      preimplantation development.
CI  - Copyright 2005 Wiley-Liss, Inc.
FAU - Cui, Xiang-Shun
AU  - Cui XS
AD  - Department of Animal Science, Chungbuk National University, Gaesin-dong, 
      Cheongju, Chungbuk, 361-763, South Korea.
FAU - Shin, Mi-Ra
AU  - Shin MR
FAU - Lee, Kyung-Ah
AU  - Lee KA
FAU - Kim, Nam-Hyung
AU  - Kim NH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blastocyst/*metabolism
MH  - Computational Biology
MH  - DNA Primers
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - Genes/*genetics
MH  - Linear Models
MH  - Mice
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sequence Analysis, DNA
EDAT- 2004/12/31 09:00
MHDA- 2005/06/09 09:00
CRDT- 2004/12/31 09:00
PHST- 2004/12/31 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2004/12/31 09:00 [entrez]
AID - 10.1002/mrd.20210 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2005 Mar;70(3):278-87. doi: 10.1002/mrd.20210.