PMID- 15626611
OWN - NLM
STAT- MEDLINE
DCOM- 20050609
LR  - 20191210
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 20
IP  - 8
DP  - 2005 Feb 15
TI  - Bioelectronic sniffers for ethanol and acetaldehyde in breath air after drinking.
PG  - 1573-9
AB  - Two kinds of bioelectronic gas sensors (bio-sniffer) incorporating alcohol 
      oxidase (AOD) and aldehyde dehydrogenase (ALDH) were developed for the convenient 
      analysis of ethanol and acetaldehyde in expired gas, respectively. The sniffer 
      devices for gaseous ethanol and acetaldehyde were constructed by immobilizing 
      enzyme on electrodes covered with filter paper and hydrophilic PTFE membrane, 
      respectively. The AOD and ALDH sniffers were used in the gas phase to measure 
      ethanol vapor from 1.0 to 500 ppm, and acetaldehyde from 0.11 to 10 ppm covering 
      the concentration range encountered in breath after alcohol consumption. Both 
      bio-sniffers displayed good gas selectivity which was attributed to the substrate 
      specificity of the relevant enzymes (AOD and ALDH) as gas recognition material. 
      From the results of physiological application, the bio-sniffers could monitor the 
      concentration changes in breath ethanol and acetaldehyde after drinking. The 
      ethanol and acetaldehyde concentrations in expired air from ALDH2 [-] (aldehyde 
      dehydrogenase type 2 negative) subjects were higher than that of the ALDH2 [+] 
      (positive) subjects. The results indicated that the lower activity of ALDH2 
      induced an adverse effect on ethanol metabolism, leading to ethanol and 
      acetaldehyde remaining in the human body, even human expired air.
FAU - Mitsubayashi, Kohji
AU  - Mitsubayashi K
AD  - Department of Biomedical Devices and Instrumentation, Institute of Biomaterials 
      and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, 
      Chiyoda-ku, Tokyo 2-3-10, Japan. m.bdi@tmd.ac.jp
FAU - Matsunaga, Hiroyuki
AU  - Matsunaga H
FAU - Nishio, Genki
AU  - Nishio G
FAU - Toda, Satoshi
AU  - Toda S
FAU - Nakanishi, Yusuke
AU  - Nakanishi Y
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 3K9958V90M (Ethanol)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.3.13 (alcohol oxidase)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase)
RN  - GO1N1ZPR3B (Acetaldehyde)
SB  - IM
MH  - Acetaldehyde/*analysis/chemistry
MH  - Adult
MH  - *Alcohol Drinking
MH  - Alcohol Oxidoreductases/analysis/chemistry
MH  - Aldehyde Dehydrogenase/analysis/chemistry
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Breath Tests/*instrumentation/methods
MH  - Electrochemistry/*instrumentation/methods
MH  - Electronics
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Ethanol/*analysis/chemistry
MH  - Humans
MH  - Male
MH  - Odorants/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2005/01/01 09:00
MHDA- 2005/06/10 09:00
CRDT- 2005/01/01 09:00
PHST- 2004/05/20 00:00 [received]
PHST- 2004/07/26 00:00 [revised]
PHST- 2004/08/04 00:00 [accepted]
PHST- 2005/01/01 09:00 [pubmed]
PHST- 2005/06/10 09:00 [medline]
PHST- 2005/01/01 09:00 [entrez]
AID - S0956-5663(04)00362-8 [pii]
AID - 10.1016/j.bios.2004.08.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2005 Feb 15;20(8):1573-9. doi: 10.1016/j.bios.2004.08.007.