PMID- 15626744
OWN - NLM
STAT- MEDLINE
DCOM- 20050513
LR  - 20220318
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 105
IP  - 8
DP  - 2005 Apr 15
TI  - The role of CXCL12 in the organ-specific process of artery formation.
PG  - 3155-61
AB  - CXC chemokine ligand 12 (CXCL12; stromal cell-derived factor-1 [SDF-1]/pre-B-cell 
      growth-stimulating factor [PBSF]) and its receptor CXCR4 are essential for 
      vascularization in the gastrointestinal tract as well as B lymphopoiesis and 
      colonization of bone marrow by hematopoietic cells. However, the mechanism by 
      which CXCL12/CXCR4 functions in blood vessel formation remains elusive. Here, we 
      have found a novel mode of organ vascularization and determined the roles of 
      CXCL12 in these processes. In the developing small intestine, many short 
      interconnecting vessels form between larger superior mesenteric artery (SMA) and 
      the neighboring primary capillary plexus surrounding the primitive gut, and they 
      elongate and become the arteries supplying the small intestine. Mice lacking 
      CXCL12 or CXCR4 lack the interconnecting vessels but have normal venous networks. 
      The mutants lack filopodial extension and intussusception from endothelial cells 
      of SMAs seen in wild-type embryos. CXCR4 is specifically expressed in arteries in 
      the developing mesenteries and its expression is severely reduced in CXCL12-/- 
      embryos. Mice in which CXCR4 is specifically deleted in the endothelium reveal 
      vascular defects identical to those observed in the conventional CXCR4-/- 
      embryos. Together, CXCL12 acts on arterial endothelial cells of SMA to 
      up-regulate CXCR4 and mediate the connection between the larger artery and 
      neighboring capillary plexus in an organ-specific manner.
FAU - Ara, Toshiaki
AU  - Ara T
AD  - Department of Medical Systems Control, Institute for Frontier Medical Sciences, 
      Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
FAU - Tokoyoda, Koji
AU  - Tokoyoda K
FAU - Okamoto, Rika
AU  - Okamoto R
FAU - Koni, Pandelakis A
AU  - Koni PA
FAU - Nagasawa, Takashi
AU  - Nagasawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041230
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Animals
MH  - Capillaries/embryology
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/*genetics/*metabolism
MH  - Endothelial Cells/physiology/ultrastructure
MH  - Endothelium, Vascular/cytology/physiology
MH  - Gene Expression Regulation, Developmental
MH  - Intestine, Small/blood supply/embryology
MH  - Mesenteric Arteries/cytology/*embryology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Pseudopodia/physiology
MH  - Receptors, CXCR4/genetics/metabolism
EDAT- 2005/01/01 09:00
MHDA- 2005/05/14 09:00
CRDT- 2005/01/01 09:00
PHST- 2005/01/01 09:00 [pubmed]
PHST- 2005/05/14 09:00 [medline]
PHST- 2005/01/01 09:00 [entrez]
AID - S0006-4971(20)45608-3 [pii]
AID - 10.1182/blood-2004-07-2563 [doi]
PST - ppublish
SO  - Blood. 2005 Apr 15;105(8):3155-61. doi: 10.1182/blood-2004-07-2563. Epub 2004 Dec 
      30.