PMID- 15627278
OWN - NLM
STAT- MEDLINE
DCOM- 20051121
LR  - 20191210
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 19
IP  - 5
DP  - 2005 Jun
TI  - Study on the bioavailability of puerarin from Pueraria lobata isoflavone 
      self-microemulsifying drug-delivery systems and tablets in rabbits by liquid 
      chromatography-mass spectrometry.
PG  - 375-8
AB  - To evaluate the bioavailability of puerarin from Pueraria lobata isoflavone 
      self-microemulsifying drug delivery systems (SMEDDS) and Yufengningxin tablets, a 
      rapid and specific liquid chromatography--mass spectrometric method was developed 
      and validated to determine puerarin in rabbit serum. The analyte was extracted 
      from serum samples by precipitating the serum proteins, separated on a Diamonsil 
      C(18) column and detected by mass spectrometry with an electrospray ionization 
      interface. 4-Hydroxybenzaldehyde was used as the internal standard. The method 
      has a limit of quantitation of 10 ng/mL using 200 microL serum. The intra-day 
      relative standard deviations (RSDs) ranged from 3.7 to 6.9% and inter-day RSDs 
      were within 6.5%. After administration of SMEDDS and tablets to rabbits, a 
      significant difference was observed in main pharmacokinetic parameters of t(max), 
      C(max) and AUC(0--infinity) between SMEDDS and tablets, and a 2.2-fold increase 
      in the relative bioavailability of puerarin was observed with the SMEDDS compared 
      with Yufengningxin tablets. It was concluded that the absorption of puerarin from 
      Pueraria lobata isoflavone SMEDDS was enhanced.
CI  - (c) 2004 John Wiley & Sons, Ltd.
FAU - Cui, Shengmiao
AU  - Cui S
AD  - School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's 
      Republic of China.
FAU - Zhao, Chunshun
AU  - Zhao C
FAU - Tang, Xing
AU  - Tang X
FAU - Chen, Dawei
AU  - Chen D
FAU - He, Zhonggui
AU  - He Z
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Emulsions)
RN  - 0 (Isoflavones)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Biological Availability
MH  - Chromatography, Liquid/methods
MH  - Drug Delivery Systems
MH  - Emulsions
MH  - Isoflavones/*pharmacokinetics
MH  - Male
MH  - Mass Spectrometry
MH  - Pueraria/*chemistry
MH  - Rabbits
EDAT- 2005/01/01 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/01/01 09:00
PHST- 2005/01/01 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/01/01 09:00 [entrez]
AID - 10.1002/bmc.460 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2005 Jun;19(5):375-8. doi: 10.1002/bmc.460.