PMID- 15627280
OWN - NLM
STAT- MEDLINE
DCOM- 20051121
LR  - 20161124
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 19
IP  - 5
DP  - 2005 Jun
TI  - Non-aqueous capillary electrophoresis for simultaneous separation and 
      determination of three major active components in traditional medicinal 
      preparations.
PG  - 369-74
AB  - A simple and sensitive non-aqueous capillary electrophoresis method has been 
      developed for simultaneous assay of three bioactive components (puerarin, 
      daidzein and wogonin) in three traditional medicinal preparations for the first 
      time. Optimum separation of the analytes was obtained on a 47 cm x 75 microm i.d. 
      capillary using a non-aqueous buffer system of 20% acetonitrile, 25 mm ammonium 
      acetate and apparent pH 9.00, with applied voltage and capillary temperature of 
      20 kV and 16 degrees C, respectively. The relative standard deviations (RSDs) of 
      the migration times and the peak areas of the three analytes were in the ranges 
      2.5--4.0% and 3.2--3.9%, respectively. Detection limits of puerarin, daidzein and 
      wogonin were 0.090, 0.145 and 0.090 microg mL(-1), respectively. In the tested 
      concentration range, good linear relationships (correlation coef fi cients: 
      0.9998 for puerarin, 0.9998 for daidzein and 0.9978 for wogonin) between peak 
      areas and concentrations of the analytes were observed. This method has been 
      successfully applied to simultaneous determination of the three bioactive 
      components with recoveries from 91.0 to 114.0%.
CI  - (c) 2004 John Wiley & Sons, Ltd.
FAU - Li, Cunhong
AU  - Li C
AD  - Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of 
      China.
FAU - Zhang, Jiyou
AU  - Zhang J
FAU - Chen, Anjia
AU  - Chen A
FAU - Xie, Jianping
AU  - Xie J
FAU - Chen, Xingguo
AU  - Chen X
FAU - Hu, Zhide
AU  - Hu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Acetates)
RN  - 0 (Acetonitriles)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavanones)
RN  - 0 (Isoflavones)
RN  - 6287WC5J2L (daidzein)
RN  - POK93PO28W (wogonin)
RN  - RRE756S6Q2 (ammonium acetate)
RN  - Y4S76JWI15 (Methanol)
RN  - Z072SB282N (acetonitrile)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Acetates
MH  - Acetonitriles
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Flavanones/*analysis/*isolation & purification
MH  - Hydrogen-Ion Concentration
MH  - Isoflavones/*analysis/*isolation & purification
MH  - Methanol
MH  - Reproducibility of Results
MH  - Temperature
EDAT- 2005/01/01 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/01/01 09:00
PHST- 2005/01/01 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/01/01 09:00 [entrez]
AID - 10.1002/bmc.459 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2005 Jun;19(5):369-74. doi: 10.1002/bmc.459.