PMID- 15627747
OWN - NLM
STAT- MEDLINE
DCOM- 20050621
LR  - 20081121
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 108
IP  - 4
DP  - 2005
TI  - High susceptibility of chromosome 16 to radiation-induced chromosome 
      rearrangements in human lymphocytes under in vivo and in vitro exposure.
PG  - 287-92
AB  - The aim of the present study was to investigate whether chromosome 16p presents 
      breakpoint regions susceptible to radiation-induced rearrangements. The 
      frequencies of translocations were determined by fluorescence in situ 
      hybridization (FISH) using cosmid probes C40 and C55 mapping on chromosome 16p, 
      and a chromosome 16 centromere-specific probe (pHUR195). Peripheral lymphocytes 
      were collected from normal individuals and from seven victims of 137Cs in the 
      Goiania (Brasil) accident (absorbed doses: 0.8-4.6 Gy) 10 years after exposure. 
      In vitro irradiated lymphocytes (3 Gy) were also analyzed. The mean translocation 
      frequency/cell obtained for the 137Cs exposed individuals was 2.4-fold higher 
      than the control value (3.6 x 10(-3) +/- 0.001), and the in vitro irradiated 
      lymphocytes showed a seven-fold increase. The genomic translocation frequencies 
      (FGs) were calculated by the formula Fp = 2.05 fp(1-fp)FG (Lucas et al., 1992). 
      For the irradiated lymphocytes and victims of 137Cs, the FGs calculated on the 
      basis of chromosome 16 were 2- to 8-fold higher than those for chromosomes 1, 4 
      and 12. Our results indicate that chromosome 16 is more prone to 
      radiation-induced chromosome breaks, and demonstrate a non-random distribution of 
      induced aberrations. This information is valuable for retrospective biological 
      dosimetry in case of human exposure to radiation, since the estimates of absorbed 
      doses are calculated by determining the translocation frequency for a sub-set of 
      chromosomes, and the results are extrapolated to the whole genome, assuming a 
      random distribution of induced aberrations. Furthermore, the demonstration of 
      breakpoints on 16p is compatible with the reports about their involvement in 
      neoplasias.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Camparoto, M L
AU  - Camparoto ML
AD  - Departamento de Genetica, Faculdade de Medicina de Ribeirao Preto, Ribeirao 
      Preto, SP, Brasil.
FAU - Takahashi-Hyodo, S A
AU  - Takahashi-Hyodo SA
FAU - Dauwerse, J G
AU  - Dauwerse JG
FAU - Natarajan, A T
AU  - Natarajan AT
FAU - Sakamoto-Hojo, E T
AU  - Sakamoto-Hojo ET
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Cesium Radioisotopes)
SB  - IM
MH  - Adult
MH  - Brazil/epidemiology
MH  - Cells, Cultured
MH  - Cesium Radioisotopes/adverse effects
MH  - Chromosome Painting/methods
MH  - Chromosomes, Human, Pair 16/*genetics/*radiation effects
MH  - Female
MH  - Gene Rearrangement/genetics/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lymphocytes/chemistry/cytology/metabolism/*radiation effects
MH  - Male
MH  - Middle Aged
MH  - Radiation Dosage
MH  - Radioactive Hazard Release
MH  - Time
MH  - Translocation, Genetic/radiation effects
EDAT- 2005/01/04 09:00
MHDA- 2005/06/23 09:00
CRDT- 2005/01/04 09:00
PHST- 2004/03/24 00:00 [received]
PHST- 2004/06/25 00:00 [accepted]
PHST- 2005/01/04 09:00 [pubmed]
PHST- 2005/06/23 09:00 [medline]
PHST- 2005/01/04 09:00 [entrez]
AID - 81522 [pii]
AID - 10.1159/000081522 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2005;108(4):287-92. doi: 10.1159/000081522.