PMID- 15627776
OWN - NLM
STAT- MEDLINE
DCOM- 20050331
LR  - 20191210
IS  - 0378-7346 (Print)
IS  - 0378-7346 (Linking)
VI  - 59
IP  - 1
DP  - 2005
TI  - Chromosomal constitution of embryos derived from tripronuclear zygotes studied by 
      fluorescence in situ hybridization using probes for chromosomes 4, 13, 18, 21, X, 
      and Y.
PG  - 14-8
AB  - This study was designed to assess the chromosomal constitution and segregation 
      patterns of cleaving embryos derived from tripronuclear zygotes. Thirty-two 
      embryos obtained from 19 conventional IVF patients were analyzed by fluorescence 
      in situ hybridization (FISH) using probes for chromosomes 4, 13, 18, 21, X, and 
      Y. Sixteen embryos (50.0%) exhibited uniform, non-mosaic patterns. These embryos 
      showed pure triploid (n = 7), pure diploid (n = 7), or pure haploid (n = 2). The 
      remaining 16 embryos showed mosaic patterns; 1 was triploid-diploid mosaics, 9 
      were diploid-haploid, and 4 were haploid only. Autosomal aneuploidy occurred in 2 
      embryos showing a triploid complement. The sex chromosomal constituent 
      XXX:XXY:XYY was 3:4:2 in embryos showing a pure triploid complement (including 2 
      embryos with aneuploidy). This ratio was not significantly different from the 
      expected 1:2:1 (p = 0.96). Pure triploid was found in 41.7% of 2-cell embryos, 
      but no triploid complement was found in 3-cell embryos. The present study also 
      supports the diandric origin of tripronuclear zygotes in the conventional IVF, 
      and, to our knowledge, is the first study to use simultaneous six-color FISH for 
      chromosomes 4, 13, 18, 21, X, and Y in human embryos. However, no additive 
      information was obtained about chromosome 4.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Pang, Myung-Geol
AU  - Pang MG
AD  - Institute of Reproductive Medicine and Population, Medical Research Center, Seoul 
      National University, Seoul, Korea.
FAU - Jee, Byung-Chul
AU  - Jee BC
FAU - Kim, Seok-Hyun
AU  - Kim SH
FAU - Ryu, Buom-Yong
AU  - Ryu BY
FAU - Oh, Sun-Kyung
AU  - Oh SK
FAU - Suh, Chang-Suk
AU  - Suh CS
FAU - Moon, Shin-Yong
AU  - Moon SY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040818
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human
MH  - Embryo, Mammalian/*physiology
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Sex Chromosomes
MH  - Zygote
EDAT- 2005/01/04 09:00
MHDA- 2005/04/01 09:00
CRDT- 2005/01/04 09:00
PHST- 2003/09/16 00:00 [received]
PHST- 2004/06/18 00:00 [accepted]
PHST- 2005/01/04 09:00 [pubmed]
PHST- 2005/04/01 09:00 [medline]
PHST- 2005/01/04 09:00 [entrez]
AID - 80522 [pii]
AID - 10.1159/000080522 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2005;59(1):14-8. doi: 10.1159/000080522. Epub 2004 Aug 18.