PMID- 15627964
OWN - NLM
STAT- MEDLINE
DCOM- 20050606
LR  - 20061115
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 5
IP  - 2
DP  - 2005 Feb
TI  - Housekeeping proteins: a preliminary study illustrating some limitations as 
      useful references in protein expression studies.
PG  - 566-71
AB  - A primary objective of many protein expression studies is to define expression 
      patterns that can distinguish between normal and diseased states, enabling a 
      better understanding of molecular events associated with disease development and 
      progression and ultimately potentially finding novel markers or therapeutic 
      targets. Exploration and confirmation of many proteins is often done using 
      Western blotting with normalization against "housekeeping proteins", such as 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, or beta-tubulin, to 
      correct for protein loading and factors, such as transfer efficiency. 
      Increasingly, in studies examining gene transcript levels, it has been shown that 
      some of the commonly used housekeeping genes may be unsuitable due to the 
      influence of various physiological and pathological factors on their expression. 
      This has not been examined to any great extent for proteins, however. This study 
      examines the degree of variability of three commonly used "housekeeping" proteins 
      (GAPDH, beta-actin, and beta-tubulin) together with class I beta-tubulin, with 
      comparisons being made between a number of different established renal cancer 
      cell lines, matched pairs of renal tumor and normal kidney lysates as well as 
      nine different human tissues and highlights some of the problems encountered.
FAU - Ferguson, Roisean E
AU  - Ferguson RE
AD  - Cancer Research UK Clinical Centre, St. James's University Hospital, UK.
FAU - Carroll, Helen P
AU  - Carroll HP
FAU - Harris, Adrian
AU  - Harris A
FAU - Maher, Eamonn R
AU  - Maher ER
FAU - Selby, Peter J
AU  - Selby PJ
FAU - Banks, Rosamonde E
AU  - Banks RE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Actins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (Tubulin)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Actins/metabolism
MH  - Blotting, Western
MH  - Carcinoma, Renal Cell/chemistry/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Humans
MH  - Kidney/chemistry/*metabolism
MH  - Kidney Neoplasms/chemistry/*metabolism/pathology
MH  - Neoplasm Proteins/chemistry/*metabolism
MH  - Proteins/chemistry/*metabolism
MH  - Reference Standards
MH  - Tubulin/metabolism
EDAT- 2005/01/04 09:00
MHDA- 2005/06/07 09:00
CRDT- 2005/01/04 09:00
PHST- 2005/01/04 09:00 [pubmed]
PHST- 2005/06/07 09:00 [medline]
PHST- 2005/01/04 09:00 [entrez]
AID - 10.1002/pmic.200400941 [doi]
PST - ppublish
SO  - Proteomics. 2005 Feb;5(2):566-71. doi: 10.1002/pmic.200400941.