PMID- 15628144
OWN - NLM
STAT- MEDLINE
DCOM- 20050502
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1059
IP  - 1-2
DP  - 2004 Dec 3
TI  - Capillary electrophoresis-based analysis of phospholipid and glycosaminoglycan 
      binding by human beta2-glycoprotein I.
PG  - 215-22
AB  - Human beta2-glycoprotein I (beta2gpI) is a phospholipid and heparin binding 
      plasma glycoprotein involved in autoimmune diseases characterized by blood 
      clotting disturbances (thrombosis) together with the occurrence of autoantibodies 
      against beta2gpI. With the final goal of assessing autoantibody influence on 
      binding interactions of beta2gpI we have studied the development of capillary 
      electrophoresis (CE)-based assays for interactions of negatively charged ligands 
      with beta2gpI. In the development of suitable conditions for analysis at neutral 
      pH of this basic protein (pI about 8) we found the pH hysteresis behavior of 
      fused silica surfaces useful since the protonated surface after an acid pre-wash 
      counteracted protein adsorption efficiently in contrast to more laborious 
      procedures including acrylamide/dimethylacrylamide coatings that did not permit 
      analysis of this particular protein. This simple approach made estimates of 
      heparin-beta2gpI interactions possible and the principle was shown also to work 
      for detection of betagpI binding to anionic phospholipids. Utilizing the pH 
      hysteresis effect may be a simple solution to the adsorption problems often 
      encountered in analyses of proteins by CE.
FAU - Bohlin, Maria E
AU  - Bohlin ME
AD  - Department of Chemistry, Karlstad University, SE-651 88 Karlstad, Sweden.
FAU - Kogutowska, Ewa
AU  - Kogutowska E
FAU - Blomberg, Lars G
AU  - Blomberg LG
FAU - Heegaard, Niels H H
AU  - Heegaard NH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Glycoproteins)
RN  - 0 (Phospholipids)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Glycoproteins/*metabolism
MH  - Glycosylation
MH  - Humans
MH  - Phospholipids/*metabolism
MH  - Protein Binding
MH  - beta 2-Glycoprotein I
EDAT- 2005/01/05 09:00
MHDA- 2005/05/03 09:00
CRDT- 2005/01/05 09:00
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/05/03 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - S0021-9673(04)01744-3 [pii]
AID - 10.1016/j.chroma.2004.09.087 [doi]
PST - ppublish
SO  - J Chromatogr A. 2004 Dec 3;1059(1-2):215-22. doi: 10.1016/j.chroma.2004.09.087.