PMID- 15629035
OWN - NLM
STAT- MEDLINE
DCOM- 20050211
LR  - 20240326
IS  - 1672-0229 (Print)
IS  - 2210-3244 (Electronic)
IS  - 1672-0229 (Linking)
VI  - 1
IP  - 3
DP  - 2003 Aug
TI  - Genome organization of the SARS-CoV.
PG  - 226-35
AB  - Annotation of the genome sequence of the SARS-CoV (severe acute respiratory 
      syndrome-associated coronavirus) is indispensable to understand its evolution and 
      pathogenesis. We have performed a full annotation of the SARS-CoV genome 
      sequences by using annotation programs publicly available or developed by 
      ourselves. Totally, 21 open reading frames (ORFs) of genes or putative 
      uncharacterized proteins (PUPs) were predicted. Seven PUPs had not been reported 
      previously, and two of them were predicted to contain transmembrane regions. 
      Eight ORFs partially overlapped with or embedded into those of known genes, 
      revealing that the SARS-CoV genome is a small and compact one with overlapped 
      coding regions. The most striking discovery is that an ORF locates on the minus 
      strand. We have also annotated non-coding regions and identified the 
      transcription regulating sequences (TRS) in the intergenic regions. The analysis 
      of TRS supports the minus strand extending transcription mechanism of 
      coronavirus. The SNP analysis of different isolates reveals that mutations of the 
      sequences do not affect the prediction results of ORFs.
FAU - Xu, Jing
AU  - Xu J
AD  - Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China.
FAU - Hu, Jianfei
AU  - Hu J
FAU - Wang, Jing
AU  - Wang J
FAU - Han, Yujun
AU  - Han Y
FAU - Hu, Yongwu
AU  - Hu Y
FAU - Wen, Jie
AU  - Wen J
FAU - Li, Yan
AU  - Li Y
FAU - Ji, Jia
AU  - Ji J
FAU - Ye, Jia
AU  - Ye J
FAU - Zhang, Zizhang
AU  - Zhang Z
FAU - Wei, Wei
AU  - Wei W
FAU - Li, Songgang
AU  - Li S
FAU - Wang, Jun
AU  - Wang J
FAU - Wang, Jian
AU  - Wang J
FAU - Yu, Jun
AU  - Yu J
FAU - Yang, Huanming
AU  - Yang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genomics Proteomics Bioinformatics
JT  - Genomics, proteomics & bioinformatics
JID - 101197608
SB  - IM
MH  - Amino Acid Substitution
MH  - Base Composition
MH  - Base Sequence
MH  - Computational Biology/methods
MH  - *Genome, Viral
MH  - Isoelectric Point
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Open Reading Frames
MH  - Severe acute respiratory syndrome-related coronavirus/*genetics
MH  - Sequence Analysis
MH  - Transcription, Genetic
PMC - PMC5172239
EDAT- 2005/01/05 09:00
MHDA- 2005/02/12 09:00
PMCR- 2016/11/28
CRDT- 2005/01/05 09:00
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/02/12 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
PHST- 2016/11/28 00:00 [pmc-release]
AID - S1672-0229(03)01028-3 [pii]
AID - 10.1016/s1672-0229(03)01028-3 [doi]
PST - ppublish
SO  - Genomics Proteomics Bioinformatics. 2003 Aug;1(3):226-35. doi: 
      10.1016/s1672-0229(03)01028-3.