PMID- 15629481
OWN - NLM
STAT- MEDLINE
DCOM- 20050526
LR  - 20171116
IS  - 0021-9975 (Print)
IS  - 0021-9975 (Linking)
VI  - 132
IP  - 1
DP  - 2005 Jan
TI  - Cutaneous papillomatosis in cattle.
PG  - 70-81
AB  - Three of four heifers housed together developed multiple cutaneous tumours in the 
      linea alba and on the teats 3 months after the application of plastic muzzle 
      plates with sharp tips to prevent mutual sucking and licking. Fibropapilloma with 
      many koilocytes but few intranuclear inclusions was diagnosed histologically. The 
      dermis showed neoplastic fibroblasts and a structureless intercellular matrix, 
      and nonpurulent vasculitis was also recorded. Immunohistochemical examination 
      with an antibody against L1 papillomavirus antigen demonstrated intranuclear 
      positivity in single cells of the granular and cornified layers and in many 
      mesenchymal cells in the fibrous parts of the tumours. CD3-positive lymphocytes 
      were present in the wall of some blood vessels, and in the dermis and epidermis. 
      Proliferating cell nuclear antigen was detected predominantly in the basal layer 
      of the epidermis and in the superficial dermis. Electron microscopy revealed 
      small intranuclear aggregates of virus particles in an epidermocyte, damage to 
      desmosomes and disorganization of cytokeratin filaments in many epidermocytes. 
      Aggregates of virus particles were revealed also in a fibroblast in the dermis. 
      In blood capillaries of the corium, acute swelling, inflammation and necrosis of 
      the endothelium were observed. By means of the polymerase chain reaction (PCR) 
      and nucleotide DNA sequencing of the PCR product, the virus was identified as 
      bovine papilloma virus type 1 (BPV 1). The presence of this virus in the tissue 
      was further confirmed by in-situ hybridization with a BPV 1 probe.
FAU - Jelinek, F
AU  - Jelinek F
AD  - Veterinary Histopathological Laboratory, Prague, Czech Republic.
FAU - Tachezy, R
AU  - Tachezy R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CD3 Complex)
RN  - 0 (DNA, Viral)
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/metabolism
MH  - Bovine papillomavirus 1/genetics/isolation & purification/ultrastructure
MH  - CD3 Complex/metabolism
MH  - Cattle
MH  - Cattle Diseases/metabolism/*pathology
MH  - DNA, Viral/analysis
MH  - Dermis/metabolism/pathology
MH  - Epidermis/metabolism/ultrastructure/virology
MH  - Female
MH  - Immunoenzyme Techniques/veterinary
MH  - In Situ Hybridization/veterinary
MH  - Leukocytes/metabolism/pathology
MH  - Lymphocytes/metabolism/pathology
MH  - Papilloma/metabolism/pathology/*veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Sequence Analysis, DNA/veterinary
MH  - Skin Neoplasms/metabolism/pathology/*veterinary
EDAT- 2005/01/05 09:00
MHDA- 2005/05/27 09:00
CRDT- 2005/01/05 09:00
PHST- 2003/12/22 00:00 [received]
PHST- 2004/07/03 00:00 [accepted]
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/05/27 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - S0021-9975(04)00080-5 [pii]
AID - 10.1016/j.jcpa.2004.07.001 [doi]
PST - ppublish
SO  - J Comp Pathol. 2005 Jan;132(1):70-81. doi: 10.1016/j.jcpa.2004.07.001.