PMID- 15629508
OWN - NLM
STAT- MEDLINE
DCOM- 20050526
LR  - 20050104
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 42
IP  - 1
DP  - 2005 Jan
TI  - Comparable functions of plasmacytoid and monocyte-derived dendritic cells in 
      chronic hepatitis C patients and healthy donors.
PG  - 61-7
AB  - BACKGROUND/AIMS: Dendritic cells (DCs) play a key role in immune responses 
      through antigen presentation and cytokine secretion. Hepatitis C virus (HCV) is 
      able to escape elimination by the immune system and often establishes a chronic 
      infection. To investigate whether DC dysfunction is involved in this process, we 
      have studied monoycte-derived DCs (Mo-DCs) and plasmacytoid DCs (pDCs), which 
      produce large amounts of IFN-alpha, from chronic HCV patients and healthy donors. 
      METHODS: We have assessed TNF-alpha and IFN-alpha production by pDCs using 
      intracellular staining after total PBMCs stimulation with unmethylated CG 
      dinucleotides (CpGs). The induction of allogeneic T cell proliferation by 
      immature Mo-DCs was measured using the MLR assay. The up-regulation of maturation 
      markers and the production of TNF-alpha in response to LPS were analyzed using 
      flow cytometry and ELISA, respectively. RESULTS: We have detected comparable 
      frequencies of pDCs producing TNF-alpha and IFN-alpha in both chronic HCV 
      patients and healthy donors and we have found that immature Mo-DCs from both 
      patients and donors similarly induce allogeneic T cell proliferation and mature 
      and secrete TNF-alpha in response to LPS. CONCLUSIONS: Our results demonstrate 
      that both pDC and Mo-DCs are not impaired in HCV infected patients.
FAU - Piccioli, Diego
AU  - Piccioli D
AD  - Immunology and Virology Department, Chiron Vaccines Research Center, via 
      Fiorentina 1, 53100 Siena, Italy.
FAU - Tavarini, Simona
AU  - Tavarini S
FAU - Nuti, Sandra
AU  - Nuti S
FAU - Colombatto, Piero
AU  - Colombatto P
FAU - Brunetto, Maurizia
AU  - Brunetto M
FAU - Bonino, Ferruccio
AU  - Bonino F
FAU - Ciccorossi, Pietro
AU  - Ciccorossi P
FAU - Zorat, Francesca
AU  - Zorat F
FAU - Pozzato, Gabriele
AU  - Pozzato G
FAU - Comar, Consuelo
AU  - Comar C
FAU - Abrignani, Sergio
AU  - Abrignani S
FAU - Wack, Andreas
AU  - Wack A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Interferon-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Donors
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Hepatitis C, Chronic/*immunology
MH  - Humans
MH  - Interferon-alpha/biosynthesis
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology
MH  - Plasma Cells/cytology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2005/01/05 09:00
MHDA- 2005/05/27 09:00
CRDT- 2005/01/05 09:00
PHST- 2004/07/20 00:00 [received]
PHST- 2004/09/14 00:00 [revised]
PHST- 2004/09/21 00:00 [accepted]
PHST- 2005/01/05 09:00 [pubmed]
PHST- 2005/05/27 09:00 [medline]
PHST- 2005/01/05 09:00 [entrez]
AID - S0168-8278(04)00454-4 [pii]
AID - 10.1016/j.jhep.2004.09.014 [doi]
PST - ppublish
SO  - J Hepatol. 2005 Jan;42(1):61-7. doi: 10.1016/j.jhep.2004.09.014.